Kubo K, Naoe T, Utsumi K R, Ishiguro Y, Ueda K, Shiku H, Yamada K
Department of Internal Medicine, Nagoya University Branch Hospital, Japan.
Br J Cancer. 1991 Jun;63(6):879-84. doi: 10.1038/bjc.1991.193.
A human tumour cell line, designated RMS-YM, was established from a childhood rhabdomyosarcoma. The monolayer cells were polygonal, round or spindle-shaped. The cells became multilayered and formed many focal piles when confluent. RMS-YM became stable with a doubling time of about 30 h and has been maintained for 104 passages to date. Tumourigenicity of the cells was confirmed by heterotransplantation into nude mice. Morphological features were similar to those of the primary tumour, and myofibrils were found by electron microscopy. The expression of desmin and human myoglobin, and high levels of striated muscle system specific enzymes were recognised. Chromosomal analysis revealed possible gene amplification in the form of homogeneously staining regions. Oncogene analysis was performed on the primary tumour and the cell line, but neither N-myc nor N-ras genes were amplified, nor were Ki-ras, Ha-ras or N-ras genes mutated at the 12th, 13th and 61st codons. The RMS-YM cell line may provide a system to identify novel genes which are amplified in rhabdomyosarcoma.
一种名为RMS-YM的人肿瘤细胞系，是从一名儿童横纹肌肉瘤中建立的。单层细胞呈多边形、圆形或梭形。细胞汇合时会形成多层并形成许多灶性堆积。RMS-YM变得稳定，倍增时间约为30小时，迄今为止已传代104次。通过异种移植到裸鼠中证实了细胞的致瘤性。形态学特征与原发性肿瘤相似，通过电子显微镜发现了肌原纤维。识别出结蛋白和人肌红蛋白的表达，以及高水平的横纹肌系统特异性酶。染色体分析揭示了以均匀染色区形式存在的可能的基因扩增。对原发性肿瘤和细胞系进行了癌基因分析，但N-myc和N-ras基因均未扩增，Ki-ras、Ha-ras或N-ras基因在第12、13和61密码子处也未发生突变。RMS-YM细胞系可能提供一个系统来鉴定在横纹肌肉瘤中扩增的新基因。
