Kato K, Mokuno K
Clin Chim Acta. 1984 Aug 31;141(2-3):169-77. doi: 10.1016/0009-8981(84)90008-1.
A sensitive sandwich enzyme immunoassay method for measurement of carbonic anhydrase III (CA-III) was established by use of purified antibodies to CA-III. The assay system consisted of polystyrene balls with immobilized antibody F(ab')2 fragments and the same antibody Fab' fragments labeled with beta-D-galactosidase from E. coli. The assay was highly sensitive and pg levels of CA-III were measureable. Coefficients of variation in within-run and between-run precision studies for serum CA-III were less than 10%. Serum CA-III levels in healthy subjects of various ages ranged from 0.8 to 24 ng/ml. Concentrations of immunoreactive CA-III in the extracts of various human tissues were also determined. Tissues composed of striated muscle contained more than 10 micrograms/mg protein of CA-III, whereas other tissues, including heart muscle, contained less than 0.5 microgram/mg protein. These results were consistent with other data showing that serum CA-III levels were raised in patients with progressive muscular dystrophy but not in those with acute myocardial infarction.
利用纯化的抗碳酸酐酶III（CA-III）抗体建立了一种灵敏的夹心酶免疫分析法来测定CA-III。该分析系统由固定有抗体F(ab')2片段的聚苯乙烯球和用来自大肠杆菌的β-D-半乳糖苷酶标记的相同抗体Fab'片段组成。该分析方法高度灵敏，能够检测到皮克水平的CA-III。血清CA-III批内和批间精密度研究的变异系数均小于10%。不同年龄健康受试者的血清CA-III水平在0.8至24 ng/ml之间。还测定了各种人体组织提取物中免疫反应性CA-III的浓度。由横纹肌组成的组织中CA-III含量超过10微克/毫克蛋白质，而其他组织，包括心肌，含量低于0.5微克/毫克蛋白质。这些结果与其他数据一致，表明进行性肌营养不良患者的血清CA-III水平升高，而急性心肌梗死患者则不然。
