Schwenkenbecher J M, Mordue A J, Piertney S B
Institute of Biological and Environmental Sciences, University of Aberdeen, Tillydrone Avenue, Aberdeen AB 24 2TZ, UK.
Bull Entomol Res. 2009 Aug;99(4):371-5. doi: 10.1017/S0007485308006391. Epub 2008 Dec 9.
Analysis of DNA sequence data has proven invaluable for defining the relationships among taxa, as well as resolving their evolutionary histories. Here, we analyzed DNA sequence variation of one mitochondrial gene (COI) and two nuclear regions (ITSI and II) to clarify the phylogenetic position of Culicoides dewulfi, a midge species widely spread in Europe and a suspected vector for bluetongue virus. Various authors have described C. dewulfi either as part of the Culicoides obsoletus sensu lato complex or as a separate taxonomic group. A maximum likelihood phylogeny, based upon an optimal model of sequence evolution, placed C. dewulfi outwith the C. obsoletus s.l. complex. Shimodaira-Hasegawa test highlighted that this topology was significantly more likely than any topology that placed C. dewulfi anywhere else in the phylogeny. As such, C. dewulfi should not be considered part of the C. obsoletus s.l. complex and instead be treated as a separate group, phylogenetically close to the classical Old World vector C. imicola.
DNA序列数据分析已被证明在确定分类单元之间的关系以及解析它们的进化历史方面具有极高价值。在此，我们分析了一个线粒体基因（COI）和两个核区域（ITSI和II）的DNA序列变异，以阐明广泛分布于欧洲且被怀疑是蓝舌病毒传播媒介的蠛蠓物种——德氏库蠓（Culicoides dewulfi）的系统发育位置。不同作者将德氏库蠓描述为广布库蠓复合种（Culicoides obsoletus sensu lato complex）的一部分，或者是一个单独的分类群。基于序列进化的最优模型构建的最大似然系统发育树将德氏库蠓置于广布库蠓复合种之外。Shimodaira - Hasegawa检验表明，这种拓扑结构比将德氏库蠓置于系统发育树其他任何位置的拓扑结构具有显著更高的可能性。因此，德氏库蠓不应被视为广布库蠓复合种的一部分，而应被视为一个单独的类群，在系统发育上与经典的旧大陆传播媒介——非洲库蠓（C. imicola）相近。
