免疫组织化学中抗原修复的最新技术。

State of the art in antigen retrieval for immunohistochemistry.

作者信息

D'Amico Fabio, Skarmoutsou Evangelia, Stivala Franca

机构信息

Department of Biomedical Sciences, University of Catania, Via Androne 83, 95124 Catania, Italy.

出版信息

J Immunol Methods. 2009 Feb 28;341(1-2):1-18. doi: 10.1016/j.jim.2008.11.007. Epub 2008 Dec 6.

DOI:10.1016/j.jim.2008.11.007

PMID:19063895

Abstract

The masking effects of antigens by chemical fixation, processing, embedding media interactions, represent a serious problem for immunohistochemical purposes. Fortunately, different approaches in antigen retrieval exist. These techniques are relatively recent and continuously expanding. This review focuses on the present state of the art in antigen retrieval methods for immunohistochemistry in light and electron microscopy. Moreover, a brief discussion on the chemical aspects of fixation, mechanism of retrieval, as well as its efficacy, is given.

摘要

化学固定、处理、包埋介质相互作用对抗原的掩盖作用，对于免疫组织化学而言是一个严重问题。幸运的是，存在不同的抗原修复方法。这些技术相对较新且在不断发展。本综述着重于光镜和电镜免疫组织化学抗原修复方法的当前技术水平。此外，还对固定的化学方面、修复机制及其效果进行了简要讨论。