Clark Susan M, De Bari Cosimo, Roelofs Anke J
Arthritis and Regenerative Medicine Laboratory, Centre for Arthritis and Musculoskeletal Health, University of Aberdeen, Aberdeen, UK.
Centre for Genomic and Experimental Medicine, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2025;2885:461-477. doi: 10.1007/978-1-0716-4306-8_24.
Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic and translational research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralized tissues is more challenging than with soft tissues. Demineralizing the samples allows embedding in paraffin wax, and also facilitates cryosectioning. This chapter describes methods for IHC on formaldehyde-fixed, demineralized, paraffin-embedded, or frozen sections to detect antigens in skeletal tissues. We include a protocol using a streptavidin-based amplification method for immunofluorescence staining.
免疫组织化学（IHC）是临床病理状况诊断以及基础研究和转化研究中常用的技术。它结合了解剖学、免疫学和生物化学方法，依赖于抗体与抗原的特异性结合。与软组织相比，将该技术用于矿化组织更具挑战性。对样品进行脱矿处理可使其嵌入石蜡，也便于冷冻切片。本章介绍了在甲醛固定、脱矿、石蜡包埋或冷冻切片上进行免疫组织化学以检测骨骼组织中抗原的方法。我们还包括一种基于链霉亲和素的免疫荧光染色扩增方法的方案。
