Wang Xian-Wei, Hu Lin, Zeng Zhu, Yao Wei-Juan, Han Jing-Yan, Zhang Ying-Yu, Xu Xiao-Feng, Liu Yu-Ying, Ka Wei-Bo, Sun Da-Gong, Wen Zong-Yao, Chien Shu
Department of Biophysics, Health Science Center, Peking University, Beijing, People's Republic of China.
Biorheology. 2008;45(5):587-98.
There is evidence that hyperlipidemia can induce hemorheological and microcirculatory disturbances. Myakuryu, a Chinese traditional medicine is efficacious in promoting lipid metabolism and protecting oxidative stress, but whether this drug can ameliorate rheologic disturbances caused by hyperlipidemia is still unknown. The present study was conducted to investigate the effects of myakuryu on hemorheological and microcirculatory disturbances induced by hyperlipidemia. Wistar rats were divided into a group on control diet (n=8) and a group on high-fat diet (HFD, n=44). Eight weeks later, plasma triglyceride (TG) and total cholesterol (TC) were determined. Sixteen animals with the highest levels of hyperlipidemia from the HFD group were randomly divided into two sub-groups: the untreated hyperlipidemia group (n=8) and the group treated with myakuryu (n=8). At the end of the sixteenth week, rheological and microcirculatory parameters were measured. Chemical analysis showed that myakuryu treatment caused significant reductions of plasma TG and TC levels (P<0.01), and the cholesterol/phospholipid ratio in the erythrocyte membrane (P<0.05). Rheological and microcirculatory measurements showed that myakuryu treatment led to a significant decrease in the erythrocyte aggregation index, plasma viscosity and blood viscosity at shear rates of 50, 100 and 150 s(-1) and in adherent leukocytes in mesenteric venules. There was a significant increase in erythrocyte deformation, electrophoretic mobility, membrane fluidity and F-actin content in the erythrocyte membrane as well as in red cell velocity in mesenteric venules. Our findings suggest that myakuryu treatment can improve blood flow and reduce adherent leukocytes in the venules of rats fed with HFD by ameliorating blood viscosity, erythrocyte deformability and aggregation, and other hemorheological characteristics.
有证据表明高脂血症可诱发血液流变学和微循环障碍。中药Myakuryu在促进脂质代谢和保护氧化应激方面具有疗效，但该药物是否能改善高脂血症引起的流变学障碍仍不清楚。本研究旨在探讨Myakuryu对高脂血症诱发的血液流变学和微循环障碍的影响。将Wistar大鼠分为对照组（n = 8）和高脂饮食组（HFD，n = 44）。八周后，测定血浆甘油三酯（TG）和总胆固醇（TC）。将高脂饮食组中高脂血症水平最高的16只动物随机分为两个亚组：未治疗的高脂血症组（n = 8）和Myakuryu治疗组（n = 8）。在第十六周结束时，测量流变学和微循环参数。化学分析表明，Myakuryu治疗可显著降低血浆TG和TC水平（P < 0.01）以及红细胞膜中的胆固醇/磷脂比率（P < 0.05）。流变学和微循环测量结果显示，Myakuryu治疗可导致红细胞聚集指数、血浆粘度以及在剪切速率为50、100和150 s(-1)时的血液粘度显著降低，并减少肠系膜小静脉中的粘附白细胞。红细胞变形性、电泳迁移率、膜流动性和红细胞膜中的F-肌动蛋白含量以及肠系膜小静脉中的红细胞速度均显著增加。我们的研究结果表明，Myakuryu治疗可通过改善血液粘度、红细胞变形性和聚集性以及其他血液流变学特性，改善高脂饮食喂养大鼠小静脉中的血流并减少粘附白细胞。
