[通过超临界流体萃取从灵芝子实体中提取三萜类化合物]
[Extraction of triterpenoids from fruiting [corrected] bodies of Ganoderma lucidum by supercritical fluid extraction].
作者信息
Song Shi-hua, Jia Xiao-bin, Chen Yan, Chen Bin, Wang Li-jing, Zhao Cheng-lei
机构信息
Jiangsu Provincial Academy of Chinese Medicine, Jiangsu Engineering Research Center for Pharmaceutical Preparation, Nanjing 210028, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Sep;33(17):2104-7.
OBJECTIVE
To optimize the extracting condition for triterpenoids from the fruits of Ganoderma lucidum by supercritical fluid extraction (SFE).
METHOD
Optimum extraction conditions were studied by orthogonal tests. The total triterpenoids were determined by ultraviolet spectrophotometry and ganoderic acid B was determined by RP-HPLC.
RESULT
The optimal extraction conditions were that the pressure 25 MPa, the temperature was 45 degrees C, the extraction time was 1.5 hour, and the ethanol was employed as modifier carrier at the volume of 3 mL x g(-1).
CONCLUSION
SFE is superior and it is feasible to extract triterpenoids from fruits of Ganoderma lucidum.
目的
通过超临界流体萃取（SFE）优化灵芝果实中三萜类化合物的提取条件。
方法
采用正交试验研究最佳提取条件。总三萜类化合物通过紫外分光光度法测定，灵芝酸B通过反相高效液相色谱法测定。
结果
最佳提取条件为压力25MPa、温度45℃、提取时间1.5小时，以乙醇作为改性剂载体，用量为3mL·g⁻¹。
结论
超临界流体萃取法具有优越性，从灵芝果实中提取三萜类化合物是可行的。
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