Aydinoglu Fatma, Yilmaz Sakir N, Coskun Banu, Daglioglu Nebile, Ogulener Nuran
Department of Pharmacology, Faculty of Medicine, Cukurova University, 01330 Adana, Turkey.
Pharmacol Rep. 2008 Sep-Oct;60(5):725-34.
The relaxation of cavernous smooth muscle is critical for inducing and maintaining a penile erection. The neurogenic- and endothelium-dependent relaxation of corpus cavernosum smooth muscle and the degenerative effect of subacute ethanol treatment on the endothelial cells of corpus cavernosum was investigated in mice. In the cavernous strips contracted with phenylephrine, electrical field stimulation (EFS), acetylcholine and exogenous nitric oxide (NO) induced relaxations in the control group. Ethanol treatment abolished the endothelium-dependent relaxations induced by acetylcholine but failed to alter the relaxation to EFS and NO. L-nitroarginine, a NO synthase inhibitor, reduced relaxations induced by EFS and acetylcholine, but not those induced by NO in control and ethanol-treated mice. L-arginine prevented the response inhibited by L-nitroarginine. ODQ, a guanylyl cyclase inhibitor, inhibited relaxations in response to EFS, NO and acetylcholine in control and ethanol-treated mice. Corpus cavernosum tissues were investigated using electron microscopy and endothelial damage was observed in ethanol-treated mice. These results suggest that ethanol impairs the endothelial function of corpus cavernosum in mouse, and it may lead to erectile dysfunction through a reduced NO release via endothelial impairment.
海绵体平滑肌的舒张对于诱导和维持阴茎勃起至关重要。在小鼠中研究了海绵体平滑肌的神经源性和内皮依赖性舒张以及亚急性乙醇处理对海绵体内皮细胞的退行性影响。在与去氧肾上腺素收缩的海绵体条带中，电场刺激（EFS）、乙酰胆碱和外源性一氧化氮（NO）在对照组中诱导舒张。乙醇处理消除了乙酰胆碱诱导的内皮依赖性舒张，但未能改变对EFS和NO的舒张反应。L-硝基精氨酸，一种NO合酶抑制剂，减少了EFS和乙酰胆碱诱导的舒张，但在对照组和乙醇处理的小鼠中未减少NO诱导的舒张。L-精氨酸阻止了L-硝基精氨酸抑制的反应。ODQ，一种鸟苷酸环化酶抑制剂，抑制了对照组和乙醇处理的小鼠中对EFS、NO和乙酰胆碱的舒张反应。使用电子显微镜对海绵体组织进行了研究，在乙醇处理的小鼠中观察到了内皮损伤。这些结果表明，乙醇损害了小鼠海绵体的内皮功能，并且它可能通过内皮损伤导致的NO释放减少而导致勃起功能障碍。
