Lun Samantha W M, Wong C K, Ko Fanny W S, Hui David S C, Lam Christopher W K
Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
J Clin Immunol. 2009 May;29(3):330-42. doi: 10.1007/s10875-008-9269-1. Epub 2008 Dec 6.
We investigated the expression profile of toll-like receptors (TLRs) and TLR ligand-activated production profile of asthma-related inflammatory cytokines in asthmatic patients. The expression of TLR1-8 on monocytes, CD4+ T helper lymphocytes, CD8+ T cytotoxic lymphocytes, CD19+ B lymphocytes, and dendritic cells, and ex vivo production of cytokines from peripheral blood mononuclear cells activated by TLR ligands were measured by flow cytometry.
Ex vivo productions of TNF-alpha, IL-10, and IL-1beta by TLR4 and TLR5 ligand LPS and flagellin were significantly lower in asthmatic patients (all P < 0.05). Expression of TLR4 and TLR5 was also found to be significantly lower in asthmatic patients when compared to that of control subjects (all P < 0.05). Therefore, the decreased activation of TLR4 and TLR5 in asthmatic patients might contribute to the immunopathological mechanisms of asthma by reducing the release of Th1 and anti-inflammatory cytokines.
我们研究了哮喘患者中Toll样受体（TLRs）的表达谱以及TLR配体激活的哮喘相关炎性细胞因子的产生情况。通过流式细胞术检测单核细胞、CD4⁺辅助性T淋巴细胞、CD8⁺细胞毒性T淋巴细胞、CD19⁺B淋巴细胞和树突状细胞上TLR1 - 8的表达，以及TLR配体激活的外周血单个核细胞的细胞因子体外产生情况。
哮喘患者中，TLR4和TLR5配体脂多糖（LPS）和鞭毛蛋白刺激外周血单个核细胞产生的肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）和白细胞介素-1β（IL-1β）显著降低（均P < 0.05）。与对照组相比，哮喘患者中TLR4和TLR5的表达也显著降低（均P < 0.05）。因此，哮喘患者中TLR4和TLR5的激活降低可能通过减少Th1和抗炎细胞因子的释放而促成哮喘的免疫病理机制。
