Jarman Pamela G, Fentress Sherri L, Katz Daniel E
DNA Unit, Delaware Office of the Chief Medical Examiner, 200 South Adams Street, Wilmington, DE 19801, USA.
J Forensic Sci. 2009 Jan;54(1):95-102. doi: 10.1111/j.1556-4029.2008.00924.x. Epub 2008 Nov 29.
Because of the inception of the FBI Regional mitochondrial DNA (mtDNA) laboratories, many do not see establishing state/local mtDNA processing laboratories as a priority. Yet there is a long-term need for mtDNA processing that will exceed the capabilities of the FBI Regional mtDNA laboratories and the few other laboratories that are currently processing mtDNA, and that need can be fulfilled by state/local laboratories. Thus, the DNA Unit of the Delaware Office of the Chief Medical Examiner (OCME-DNA Unit) completed validation of in-house mtDNA testing in January 2007. The validation plan for mtDNA processing included the following sections: preliminary research, sensitivity and contamination studies, ExoSAP-IT optimization, BigDye optimization, sequencing and 310 optimization, sample preparation and extraction optimization, heteroplasmy, mixtures, and reproducibility. All sections of the validation were successfully completed, and mtDNA processing of skeletal remains, teeth, and hairs, as well as blood and buccal reference samples was adopted by the OCME-DNA Unit.
由于联邦调查局（FBI）地区线粒体DNA（mtDNA）实验室的设立，许多人认为没有必要优先建立州/地方mtDNA检测实验室。然而，对mtDNA检测的长期需求将超过FBI地区mtDNA实验室以及目前少数其他从事mtDNA检测的实验室的能力，而这种需求可以由州/地方实验室来满足。因此，特拉华州首席法医办公室DNA部门（OCME-DNA部门）于2007年1月完成了内部mtDNA检测的验证。mtDNA检测的验证计划包括以下部分：初步研究、灵敏度和污染研究、ExoSAP-IT优化、BigDye优化、测序和310优化、样本制备和提取优化、异质性、混合样本以及重现性。验证的所有部分均已成功完成，OCME-DNA部门采用了对骨骼遗骸、牙齿、毛发以及血液和口腔参考样本的mtDNA检测。
