Duddukuri Govinda Rao, Brahmam A Naga, Rao D N
Department of Biochemistry, GITAM University, Visakhapatnam 530 045, Andhra Pradesh, India.
Indian J Biochem Biophys. 2008 Oct;45(5):341-4.
Strychnos nux-vomica Linn. (SNV; Loganiaceae), a medicinal plant has been used as folk medicine for alleviating inflammation, joint pains and allergic symptoms. In the present study, we examined its possible immunomodulatory effect on induction of ovalbumin (OVA)-specific IgE antibody response in a murine model, as evaluated by passive cutaneous anaphylaxis (PCA). The OVA-specific IgE antibody response was significantly suppressed in BALB/c mice (H-2d), following intraperitoneal administration of aqueous stem extract of the plant along with OVA. Furthermore, the different doses of SNV extract were found to significantly suppress the induction of OVA-specific IgE antibody response. The anti-OVA IgE antibody response was suppressed in different haplotypes of mice viz., C57BL/6 (H-2b) and SWR/J (H-29). However, preliminary findings revealed no significant change in the total IgG antibody response against OVA, as evaluated by ELISA. These results confirm the suppressive activity of S. nux-vomica on allergen-specific IgE antibody response and suggest its possible application in allergic conditions.
Strychnlos nux-vomica, Immunomodulation, Immunosuppression, IgE antibody response, Passive cutaneous anaphylaxis, ELISA
马钱子（马钱科），一种药用植物，一直被用作民间药物来缓解炎症、关节疼痛和过敏症状。在本研究中，我们通过被动皮肤过敏反应（PCA）评估了其对小鼠模型中卵清蛋白（OVA）特异性IgE抗体反应诱导的可能免疫调节作用。在BALB/c小鼠（H-2d）中，腹腔注射该植物的水茎提取物和OVA后，OVA特异性IgE抗体反应受到显著抑制。此外，发现不同剂量的马钱子提取物能显著抑制OVA特异性IgE抗体反应的诱导。在不同单倍型的小鼠，即C57BL/6（H-2b）和SWR/J（H-29）中，抗OVA IgE抗体反应也受到抑制。然而，初步研究结果显示，通过ELISA评估，针对OVA的总IgG抗体反应没有显著变化。这些结果证实了马钱子对变应原特异性IgE抗体反应的抑制活性，并表明其在过敏病症中的可能应用。
马钱子；免疫调节；免疫抑制；IgE抗体反应；被动皮肤过敏反应；ELISA
Zotero 插件