多胺和硝酸银对葫芦（葫芦科丝瓜属；亚洲种）子叶外植体高频再生的影响。

Effect of polyamines and silver nitrate on the high frequency regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria; sp. asiatica).

作者信息

Shyamali Saha, Hattori Kazumi

机构信息

Laboratory of Plant Genetics and Breeding, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.

出版信息

Pak J Biol Sci. 2007 Apr 15;10(8):1288-93. doi: 10.3923/pjbs.2007.1288.1293.

DOI:10.3923/pjbs.2007.1288.1293

PMID:19069930

Abstract

In this study, we have investigated the effect ofpolyamines (PA) and silver nitrate (AgNO3) on the high frequency regeneration from cotyledon explants of bottle gourd containing Murashige and Skoog (MS) media supplemented with different kind of Cytokinin alone or in the combination. Synergistic effect of kinetin (1 mg L(-1)) and benzyl adenine (BA) (2 mg L(-1)) itself showed highest shoot regeneration (80.6%) efficiency than BA or Kinetin alone in cotyledon explants of bottle gourd without adding AgNO3 or PAs. We have also observed that PAs and AgNO3 show their sensitivity on the regeneration, which is hormonal dependent. Regenerated shoots were rooted in half strength MS media containing 0.1 mg L(-1) IAA.

摘要

在本研究中，我们研究了多胺（PA）和硝酸银（AgNO₃）对含有添加了不同种类细胞分裂素单独或组合的Murashige和Skoog（MS）培养基的葫芦子叶外植体高频再生的影响。在不添加AgNO₃或多胺的葫芦子叶外植体中，激动素（1 mg L⁻¹）和苄基腺嘌呤（BA）（2 mg L⁻¹）自身的协同作用显示出比单独使用BA或激动素更高的芽再生（80.6%）效率。我们还观察到多胺和AgNO₃对再生表现出敏感性，这是激素依赖性的。再生芽在含有0.1 mg L⁻¹吲哚乙酸（IAA）的1/2强度MS培养基中生根。