基因型和硝酸银对油菜（甘蓝型油菜）冬季品种茎尖再生的影响。

Effects of genotype and AgNO3 on shoot regeneration in winter cultivars of rapeseed (Brassica napus).

作者信息

Uliaie E D, Farsi M, Ghreyazie B, Imani J

机构信息

Department of Biotechnology and Plant Breeding, College of Agriculture, Ferdowsi University of Mashhad, P.O. Box 9177948978, Mashhad, Iran.

出版信息

Pak J Biol Sci. 2008 Aug 15;11(16):2040-3. doi: 10.3923/pjbs.2008.2040.2043.

DOI:10.3923/pjbs.2008.2040.2043

PMID:19266914

Abstract

Ability of shoot regeneration from cotyledon explants of rapeseed (Brassica napus) winter genotypes and the effect of silver nitrate and naphthalene acetic acid in shoot regeneration were investigated. The optimum medium for regeneration was the medium supplemented with 3 mg L(-1) 6-Benzylaminopurine and 0.15 mg L(-1) 1-naphthaleneacetic acid. The addition of 5 mg L(-1) silver nitrate significantly improved shoot regeneration. Shoot regeneration response was strongly different between genotypes with a range of variation from 79% in spring genotype PF and 7% in Okpi. The highest root production was recorded on medium containing indol-3-butyric acid. The rooted plants successfully transferred to soil and adapted to greenhouse conditions. No abnormally was observed and the regenerated plants were morphologically similar to the field grown parental plants.

摘要

研究了油菜（甘蓝型油菜）冬季基因型子叶外植体的芽再生能力以及硝酸银和萘乙酸对芽再生的影响。再生的最佳培养基是添加3 mg L(-1) 6-苄基腺嘌呤和0.15 mg L(-1) 1-萘乙酸的培养基。添加5 mg L(-1)硝酸银显著提高了芽再生。不同基因型之间的芽再生反应差异很大，变异范围从春季基因型PF的79%到Okpi的7%。在含有吲哚-3-丁酸的培养基上记录到最高的生根率。生根的植株成功移栽到土壤中并适应了温室条件。未观察到异常情况，再生植株在形态上与田间种植的亲本植株相似。