硝酸银促进了绿豆（Vigna mungo (L.) Hepper）高频芽再生。
AgNO3 boosted high-frequency shoot regeneration in Vigna mungo (L.) Hepper.
作者信息
Mookkan Muruganantham, Andy Ganapathi
机构信息
a Department of Biotechnology and Genetic Engineering; School of Biotechnology ; Bharathidasan University ; Tiruchirappalli , Tamil Nadu , India.
出版信息
Plant Signal Behav. 2014;9(10):e972284. doi: 10.4161/psb.32165.
In order to further increase shoot regeneration frequency of Vigna mungo (L.) Hepper., the effects of AgNO3 on this process was investigated in this study. The shoot tip and cotyledonary node explants were cultured on MS salts B5 Vitamins medium containing BA+TDZ+Ads+AgNO3 for multiple shoot induction. AgNO3 influenced the shoot bud formation and their subsequent proliferation. The best medium composition for multiple shoot induction was BA, TDZ combination with Ads and AgNO3 in MSB5 medium. Maximum 39 shoots in cotyledonary node and 22 shoots in shoot tip were obtained per explants after 4 - 6 wk. of culture. Elongation and rooting were performed in GA3 (0.6mg/l) and IBA (0.4mg/L) containing media respectively. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival rate of 78%.
为了进一步提高绿豆（Vigna mungo (L.) Hepper.）的芽再生频率，本研究探讨了硝酸银（AgNO3）对该过程的影响。将茎尖和子叶节外植体接种在添加了BA+TDZ+吸附剂+硝酸银的MS盐B5维生素培养基上进行多芽诱导。硝酸银影响了芽的形成及其后续增殖。多芽诱导的最佳培养基配方是在MSB5培养基中使用BA、TDZ与吸附剂和硝酸银组合。培养4-6周后，每个外植体在子叶节中最多可获得39个芽，在茎尖中最多可获得22个芽。分别在含有GA3（0.6mg/l）和IBA（0.4mg/L）的培养基中进行伸长和生根培养。离体培养的植株在温室中驯化，并成功移植到田间，成活率为78%。
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