铜绿假单胞菌RB 28产生的生物表面活性剂的乳化特性

Emulsification properties of biosurfactant produced from Pseudomonas aeruginosa RB 28.

作者信息

Sifour Mohamed, Al-Jilawi Majid H, Aziz Ghazi M

机构信息

Department of Biotechnology, College of Science, University of Baghdad, Iraq.

出版信息

Pak J Biol Sci. 2007 Apr 15;10(8):1331-5. doi: 10.3923/pjbs.2007.1331.1335.

DOI:10.3923/pjbs.2007.1331.1335

PMID:19069939

Abstract

Biosurfactant produced from P. aeruginosa RB 28 was extracted, purified and characterized. Thin layer chromatography results showed that the extract contained two different compounds. The identification of the nature of the two compounds showed that they were glycolipids and rhamnose was the sugar moiety in these glycolipids. It was concluded that these compounds were rhamnolipids. The production of biosurfactant was started at late log phase and reached its maximal level (2.7 g L(-1)) at the stationary phase. Study of some rhamnolipid properties showed that sunflower oil, heptadecane and paraffin were efficiently emulsified and emulsions formed with vegetable oils (olive oil, corn oil and sunflower oil) were more stable than emulsions formed with hydrocarbons.

摘要

从铜绿假单胞菌RB 28中产生的生物表面活性剂被提取、纯化并进行了表征。薄层色谱结果表明提取物含有两种不同的化合物。对这两种化合物性质的鉴定表明它们是糖脂，并且鼠李糖是这些糖脂中的糖部分。得出的结论是这些化合物是鼠李糖脂。生物表面活性剂的产生在对数后期开始，并在稳定期达到其最高水平（2.7 g L(-1)）。对一些鼠李糖脂性质的研究表明，向日葵油、十七烷和石蜡能被有效乳化，并且与植物油（橄榄油、玉米油和向日葵油）形成的乳液比与碳氢化合物形成的乳液更稳定。