Renju Gopi Lilly, Manoharan Shanmugam, Balakrishnan Subramanian, Senthil Namasivayam
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
Pak J Biol Sci. 2007 May 1;10(9):1465-70. doi: 10.3923/pjbs.2007.1465.1470.
The present study has investigated the chemopreventive and antilipidperoxidative effects of the ethanolic extract of Clerodendron inerme leaves (CiELet) in DMBA induced skin carcinogenesis in Swiss albino mice. The skin squamous cell carcinoma was induced in the shaved back of mice, by painting with DMBA (25 microg 0.1 mL(-1) acetone) twice weekly for 8 weeks. We have observed 100% tumor formation in the fifteenth week of experimental period. Elevated lipid peroxidation and decline in enzymatic and non-enzymatic antioxidants status was observed in tumor bearing mice. Oral administration of CiELet (300 mg kg(-1) bw) for 25 weeks significantly prevented the tumor incidence, volume and burden of the tumor. The CiELet also showed potent antilipidperoxidative effect as well as enhanced the antioxidant defense mechanisms in DMBA painted mice. The present study thus demonstrated the chemopreventive and antilipidperoxidative efficacy of CiELet in DMBA induced mouse skin carcinogenesis.
本研究调查了臭茉莉叶乙醇提取物（CiELet）在二甲基苯并蒽（DMBA）诱导的瑞士白化小鼠皮肤癌发生过程中的化学预防和抗脂质过氧化作用。通过每周两次用DMBA（25微克/0.1毫升丙酮）涂抹小鼠背部剃毛处，持续8周，诱导皮肤鳞状细胞癌。在实验期的第15周，我们观察到肿瘤形成率为100%。在荷瘤小鼠中观察到脂质过氧化升高，酶促和非酶促抗氧化剂状态下降。口服CiELet（300毫克/千克体重）25周可显著预防肿瘤发生率、肿瘤体积和肿瘤负荷。CiELet还显示出强大的抗脂质过氧化作用，并增强了DMBA涂抹小鼠的抗氧化防御机制。因此，本研究证明了CiELet在DMBA诱导的小鼠皮肤癌发生中的化学预防和抗脂质过氧化功效。
