Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India.
Environ Toxicol Pharmacol. 2009 Jul;28(1):11-8. doi: 10.1016/j.etap.2009.01.008. Epub 2009 Feb 3.
The chemopreventive potential of orally administered piperine was studied in Swiss albino mice against 7,12-dimethylbenz[a]anthracene (DMBA)-induced skin carcinogenesis. The mechanistic pathway for the chemopreventive potential of piperine was evaluated by analysing the status of phase I and phase II detoxification agents, lipid peroxidation by-products and antioxidants during DMBA-induced skin carcinogenesis. Skin squamous cell carcinoma was induced in the shaved back of mice, by painting with DMBA (25μg in 0.1ml acetone/mouse) two times weekly for 8 weeks. We observed severe hyperplasia, dysplasia, and well-differentiated squamous cell carcinoma in the 8th, 10th and 15th week of experimental period respectively in mice treated with DMBA alone. Marked alterations in the status of phase I and phase II detoxification agents, lipid peroxidation by-products and antioxidants were observed in tumor bearing mice. Oral administration of piperine (50mgkg(-1) body weight) by gastric gavage significantly prevented the formation of skin tumors during DMBA-induced mouse skin carcinogenesis. Also, piperine administration brought back the status of phase I and phase II detoxification agents, lipid peroxidation by-products and antioxidants to near normal range in DMBA treated mice. The present study thus demonstrates that piperine has significant suppressing effect on cell proliferation during DMBA-induced mouse skin carcinogenesis. The chemopreventive potential of piperine is probably due to its modulating effect on the status of lipid peroxidation, antioxidants and detoxification agents during DMBA-induced skin carcinogenesis.
胡椒碱经口给药对 7,12-二甲基苯并蒽（DMBA）诱导的皮肤癌变的化学预防作用在瑞士白化小鼠中进行了研究。通过分析 DMBA 诱导皮肤癌变过程中 I 相和 II 相解毒剂、脂质过氧化产物和抗氧化剂的状态，评估了胡椒碱化学预防作用的机制途径。通过每周两次在剃光的背部涂抹 DMBA（25μg/0.1ml 丙酮/只），诱导小鼠皮肤鳞状细胞癌。我们观察到单独用 DMBA 处理的小鼠在第 8、10 和 15 周的实验期分别出现严重的增生、发育不良和分化良好的鳞状细胞癌。在荷瘤小鼠中观察到 I 相和 II 相解毒剂、脂质过氧化产物和抗氧化剂的状态发生了明显的改变。胡椒碱（50mgkg(-1)体重）经胃管给药可显著抑制 DMBA 诱导的小鼠皮肤癌变过程中皮肤肿瘤的形成。此外，胡椒碱给药使 DMBA 处理的小鼠中 I 相和 II 相解毒剂、脂质过氧化产物和抗氧化剂的状态恢复到接近正常范围。因此，本研究表明胡椒碱对 DMBA 诱导的小鼠皮肤癌变过程中的细胞增殖具有显著的抑制作用。胡椒碱的化学预防作用可能与其在 DMBA 诱导的皮肤癌变过程中对脂质过氧化、抗氧化剂和解毒剂状态的调节作用有关。
