Manoharan S, Selvan M Vasantha
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, India.
J Environ Biol. 2012 Mar;33(2):255-60.
The present study investigated the chemopreventive potential of geraniol, an acyclic monoterpene alcohol, by monitoring the tumor incidence and analyzing the status of phase II detoxification agents, lipid peroxidation by products and antioxidants in 7,12-dimethylbenz(a)anthracene (DMBA) induced mouse skin carcinogenesis. Skin tumor was developed by painting DMBA (25 microg in 0.1 ml acetone mouse(-1)) in the shaved back of the mice, twice weekly for 8 weeks. We noticed 100% skin tumor formation in mice treated with DMBA alone. The status of phase II detoxification agents and antioxidants were decreased where as lipid peroxidation by products were increased in tumor bearing mice. Oral administration of geraniol at a dose of 250 mg kg(-1) body weight significantly prevented the tumor formation as well as brought back the status of phase II detoxification agents, lipid peroxidation by products and antioxidants to near normal range in DMBAtreated mice. Present results suggest that geraniol might have inhibited abnormal cell proliferation occurring in skin carcinogenesis by modulating the activities of phase II detoxification agents and through its free radical scavenging potential.
本研究通过监测肿瘤发生率，并分析7,12-二甲基苯并(a)蒽（DMBA）诱导的小鼠皮肤癌发生过程中II期解毒剂、脂质过氧化产物和抗氧化剂的状态，研究了无环单萜醇香叶醇的化学预防潜力。通过在小鼠背部剃毛处涂抹DMBA（25微克/0.1毫升丙酮/只小鼠）来诱发皮肤肿瘤，每周两次，共8周。我们注意到单独用DMBA处理的小鼠中100%形成了皮肤肿瘤。在荷瘤小鼠中，II期解毒剂和抗氧化剂的状态降低，而脂质过氧化产物增加。以250毫克/千克体重的剂量口服香叶醇可显著预防肿瘤形成，并使DMBA处理小鼠的II期解毒剂、脂质过氧化产物和抗氧化剂的状态恢复到接近正常范围。目前的结果表明，香叶醇可能通过调节II期解毒剂的活性及其自由基清除潜力，抑制皮肤癌发生过程中出现的异常细胞增殖。
