Dehghani M, Nasseri S, Amin S, Naddafee K, Taghavi M, Yunesian M, Maleky N
Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Pak J Biol Sci. 2007 Jan 1;10(1):84-9. doi: 10.3923/pjbs.2007.84.89.
In this study several agricultural fields with a long history of atrazine application in Fars province of Iran have been explored for their potential of atrazine biodegradation. After several subculturing for a period of 300 days acclimation, leads to an enhancement of atrazine biodegradation rate. A successful enrichment culture with a high capability for atrazine degradation was obtained (88%). A combination of enrichment culture technique, in a basal salt medium containing atrazine and carbon sources under nitrogen limitation and plating on indicator atrazine agar, have permitted the isolation of bacterial consortium with high capability of using atrazine as a nitrogen source. Seven gram-negative and one gram-positive bacterial strain, which were able to use this herbicide as a sole source of nitrogen, were isolated from Darehasalouie Kavar corn field soil. Based on physiological, biochemical and nutritional characteristics, the isolated bacteria were identified as Pseudomonas alcaligenes, Acidovorax sp., Pseudomonas putida, Ralstonia eutrophus, Pseudomonas syiringe, Erwinia tracheiphila, Entrobacter agglomerans and Micrococcus varians. Therefore, the bacterial consortium in liquid culture containing carbon sources and atrazine as a sole source of nitrogen, degrade added atrazine more than 80%.
在本研究中，对伊朗法尔斯省长期使用阿特拉津的几个农业田地进行了探索，以研究其阿特拉津生物降解潜力。经过300天的驯化和多次传代培养后，阿特拉津的生物降解率得到提高。获得了一种对阿特拉津具有高降解能力的成功富集培养物（88%）。采用富集培养技术，在含阿特拉津和碳源的基础盐培养基中，在氮限制条件下培养，并接种于指示性阿特拉津琼脂平板上，从而分离出具有高能力利用阿特拉津作为氮源的细菌群落。从达雷哈萨卢伊卡瓦尔玉米田土壤中分离出7株革兰氏阴性菌和1株革兰氏阳性菌，它们能够将这种除草剂作为唯一氮源。根据生理、生化和营养特性，将分离出的细菌鉴定为产碱假单胞菌、嗜酸菌属、恶臭假单胞菌、真养产碱杆菌、丁香假单胞菌、嗜气管欧文氏菌、成团肠杆菌和变异微球菌。因此，在含有碳源和作为唯一氮源的阿特拉津的液体培养物中的细菌群落，能将添加的阿特拉津降解80%以上。
