Kumar Anup, Singh Neera
Division of Agricultural Chemicals, ICAR - Indian Agricultural Research Institute, New Delhi, 110012, India.
Environ Monit Assess. 2016 Mar;188(3):142. doi: 10.1007/s10661-016-5144-3. Epub 2016 Feb 3.
An atrazine-degrading enrichment culture was used to study degradation of atrazine metabolites viz. hydroxyatrazine, deethylatrazine, and deisopropylatrazine in mineral salts medium. Results suggested that the enrichment culture was able to degrade only hydroxyatrazine, and it was used as the sole source of carbon and nitrogen. Hydroxyatrazine degradation slowed down when sucrose and/or ammonium hydrogen phosphate were supplemented as the additional sources of carbon and nitrogen, respectively. The enrichment culture could degrade high concentrations of atrazine (up to 110 μg/mL) in mineral salts medium, and neutral pH was optimum for atrazine degradation. Further, except in an acidic soil, enrichment culture was able to degrade atrazine in three soil types having different physico-chemical properties. Raising the pH of acidic soil to neutral or alkaline enabled the enrichment culture to degrade atrazine suggesting that acidic pH inhibited atrazine-degrading ability. The study suggested that the enrichment culture can be successfully utilized to achieve complete degradation of atrazine and its persistent metabolite hydroxyatrazine in the contaminated soil and water.
使用一种降解莠去津的富集培养物来研究莠去津代谢物即羟基莠去津、脱乙基莠去津和脱异丙基莠去津在矿物盐培养基中的降解情况。结果表明，该富集培养物仅能降解羟基莠去津，且羟基莠去津被用作碳源和氮源的唯一来源。当分别添加蔗糖和/或磷酸氢铵作为额外的碳源和氮源时，羟基莠去津的降解速度减缓。该富集培养物能够在矿物盐培养基中降解高浓度的莠去津（高达110μg/mL），中性pH值最有利于莠去津的降解。此外，除了在酸性土壤中，该富集培养物能够在三种具有不同理化性质的土壤类型中降解莠去津。将酸性土壤的pH值提高到中性或碱性可使富集培养物降解莠去津，这表明酸性pH值抑制了莠去津的降解能力。该研究表明，该富集培养物可成功用于实现污染土壤和水中莠去津及其持久性代谢物羟基莠去津的完全降解。
