Demir K O K
Department of Horticulture, Faculty of Agriculture, Namik Kemal University, Tekirdag, Turkey.
Pak J Biol Sci. 2007 Aug 15;10(16):2631-8. doi: 10.3923/pjbs.2007.2631.2638.
This research was carried out under the laboratory conditions of Department of Horticulture, Agriculture Faculty, Namik Kemal University in Turkey. In this research, salinity tolerance of V. vinifera subsp. sylvestris (C.C. Gmelin) ecotypes derived from Marmara Region and Akdeniz Region of Turkey was evaluated for various salinity levels at seed germination and plantlet stages. In addition to V. vinifera subsp. sylvestris (C.C. Gmelin); 5 BB Kober (V. berlandieri Planch. x V. riparia Michx.) and Isabella grape (V. labrusca L.) were also inserted to study to make a comparison. In order to determine responses of V. vinifera subsp. sylvestris (C.C. Gmelin) ecotypes, 5 BB Kober and Isabella grape to salinity, NaCl was added at 0 (control), 2.7, 5.4, 8.1 and 10.8 dS m(-1) to nutrient solution to achieve five salinity levels. Prior to study, all seeds were extracted from grape berries and stratified to be permeable to water by humidified sand. Afterwards, seeds were germinated under the different salinity stress conditions mentioned above. At the end of germination phases, germination percentages were calculated for all seed types and fresh weight (mg), dry weight (mg), water content (%), tolerance index values, Na+:K+ values were found out for shoots and roots of all plantlet types. No germination was observed during the germinations of all seeds under the stress conditions induced by 10.8 dS m(-1) NaCl treatment. On the basis of various salinity tolerance indexes, it was seen that Marmara Region plantlets were more resistant than Akdeniz Region for 8.1 dS m(-1) NaCl treatment. In conclusion, since V. vinifera subsp. sylvestris (C.C. Gmelin) ecotype from Marmara Region exhibits higher resistance to salinity, its rooted plant materials as grapevine rootstock can be used for salinity soil conditions in grape growing. Besides, it can be utilized from seeds of Marmara Region to obtain salinity resistant hybridized grapevine rootstocks in breeding programs of viticulture.
本研究在土耳其纳米克·凯末尔大学农学院园艺系的实验室条件下进行。在本研究中,对源自土耳其马尔马拉地区和地中海地区的野生葡萄(V. vinifera subsp. sylvestris (C.C. Gmelin))生态型在种子萌发和幼苗阶段的不同盐度水平下的耐盐性进行了评估。除了野生葡萄(V. vinifera subsp. sylvestris (C.C. Gmelin))外,还引入了5BB考贝(V. berlandieri Planch. x V. riparia Michx.)和伊莎贝拉葡萄(V. labrusca L.)进行研究以作比较。为了确定野生葡萄(V. vinifera subsp. sylvestris (C.C. Gmelin))生态型、5BB考贝和伊莎贝拉葡萄对盐度的反应,向营养液中添加氯化钠,使其浓度分别为0(对照)、2.7、5.4、8.1和10.8 dS m(-1),以达到五个盐度水平。在研究之前,所有种子均从葡萄浆果中取出,并通过湿沙分层处理以使种子透水。之后,将种子在上述不同盐度胁迫条件下萌发。在萌发阶段结束时,计算所有种子类型的发芽率,并测定所有幼苗类型地上部分和根系的鲜重(mg)、干重(mg)、含水量(%)、耐盐指数值、Na+:K+值。在10.8 dS m(-1)氯化钠处理诱导的胁迫条件下,所有种子萌发期间均未观察到发芽现象。基于各种耐盐指数,发现在8.1 dS m(-1)氯化钠处理下,马尔马拉地区的幼苗比地中海地区的更耐盐。总之,由于来自马尔马拉地区的野生葡萄(V. vinifera subsp. sylvestris (C.C. Gmelin))生态型对盐度表现出更高的抗性,其作为葡萄砧木的生根植物材料可用于葡萄种植的盐渍土壤条件。此外,在葡萄栽培育种计划中,可利用马尔马拉地区的种子来获得耐盐杂交葡萄砧木。