Wen Tao, Zhao Xia, Luo Guoan, Wang Jian, Wang Yiming, Yao Bo, Zhao Jiyuan, Zhu Jun, Yu Zhongshan
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Talanta. 2007 Feb 15;71(2):854-60. doi: 10.1016/j.talanta.2006.05.051. Epub 2006 Jun 23.
Microemulsion electrokinetic chromatography (MEEKC) and solvent modified micellar electrokinetic chromatography (MEKC) were investigated with the goal of the rapid separation of complex heroin and amphetamine samples. The rapid simultaneous separation of 17 species of heroin, amphetamine and their basic impurities and adulterants was performed within about 10min using MEEKC for the first time, whereas solvent modified MEKCs were unable to resolve all the components. The comparisons between MEEKC and solvent modified MEKC proved internal lipophilic organic phase in microemulsions played an important role in improving the separation performance with respect to efficiency. However, the role of internal lipophilic organic phase in MEEKC was disgusted at high concentrations of cosurfactant, and the separations of MEEKC and 1-butanol modified MEKC became similar at high concentrations of 1-butanol. The evaluation of reproducibility, linearity and detection limit of optimized MEEKC method provided good results for all the analytes investigated, thus allowing its application to real controlled drug preparation analysis.
为了快速分离复杂的海洛因和苯丙胺样品，对微乳液电动色谱法（MEEKC）和溶剂改性胶束电动色谱法（MEKC）进行了研究。首次使用MEEKC在约10分钟内实现了17种海洛因、苯丙胺及其碱性杂质和掺假物的快速同时分离，而溶剂改性的MEKC无法分离所有组分。MEEKC与溶剂改性MEKC之间的比较证明，微乳液中的内亲脂性有机相对提高分离效率起着重要作用。然而，在高浓度助表面活性剂的情况下，MEEKC中内亲脂性有机相的作用受到影响，在高浓度正丁醇的情况下，MEEKC和正丁醇改性MEKC的分离效果变得相似。对优化后的MEEKC方法的重现性、线性和检测限的评估为所有研究的分析物提供了良好的结果，从而使其能够应用于实际的管制药物制剂分析。
