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[脱氧核糖核酸酶I对细菌生长的影响:取决于培养条件及接种物的生理特性]

[The effect of DNAse I on bacterial growth depending on the cultivation conditions and on the physiological characteristics of the inoculate].

作者信息

Egorov C Iu, Kupriianova F G, Khaĭbullina S A, Kolpakov A I

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1991(4):109-16.

PMID:1907205
Abstract

The stimulating effect of DNAase 1 on Escherichia coli reproduction has been studied depending on the content of slowly growing cells in the inoculation culture in the phase of delayed growth. Three cell fractions of E. coli have been obtained using the stepwise separation of the population in terms of buoyant density in the phase of delayed growth. In contrast to fractions I and II the fraction III contains cells with delayed growth, permeable to exogenous DNAase 1 and sensitive to the action of this enzyme. The faster growth of bacterial cells has been shown to be caused by the direct ferment action on the slowly growing cells. The autometabolites and autolysates don't take part in this process.

摘要

已根据延迟生长阶段接种培养物中缓慢生长细胞的含量,研究了脱氧核糖核酸酶1对大肠杆菌繁殖的刺激作用。在延迟生长阶段,根据浮力密度对群体进行逐步分离,获得了大肠杆菌的三个细胞组分。与组分I和II相比,组分III含有生长延迟的细胞,对外源脱氧核糖核酸酶1具有通透性,且对该酶的作用敏感。已表明细菌细胞的更快生长是由对缓慢生长细胞的直接发酵作用引起的。自身代谢产物和自溶产物不参与这一过程。

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1
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Nauchnye Doki Vyss Shkoly Biol Nauki. 1991(4):109-16.
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