Adam Martin, Dobiás Petr, Eisner Ales, Ventura Karel
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
J Sep Sci. 2009 Jan;32(2):288-94. doi: 10.1002/jssc.200800543.
The analytical method based on the HPLC coupled with UV detection (HPLC-UV) for the determination of selected antioxidants (i.e. esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. Two ultrasonic extraction methods for the isolation of these compounds from the plants such as Mentha longifolia L., Mentha spicata L., Ruta graveolens L., Achyllea millefolium L., Plantago lanceolata L. and Coriandrum sativum L. were used. Both of these methods, i.e. ultrasonic probe and ultrasonic bath, were optimised and compared to each other. For the proposed HPLC-UV method LOQ values in the range from 22.7 (xanthotoxin) up to 97.2 ng/mL (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical was also determined. Obtained results ranged from 10.11 up to 73.50% of DPPH radical inhibition.
建立了基于高效液相色谱-紫外检测（HPLC-UV）的分析方法，用于测定植物材料中选定的抗氧化剂（即七叶亭、东莨菪素、7-羟基香豆素、芦丁、花椒毒素、5-甲氧基补骨脂素和槲皮素）。采用两种超声提取方法从长叶薄荷、留兰香、芸香、千叶蓍、披针叶车前和芫荽等植物中分离这些化合物。对超声探头和超声浴这两种方法进行了优化并相互比较。对于所提出的HPLC-UV方法，获得的定量限（LOQ）值范围为22.7（花椒毒素）至97.2 ng/mL（芦丁）。还测定了所有提取物基于还原游离2,2-二苯基-1-苦基肼（DPPH）自由基的抗氧化能力。获得的结果范围为DPPH自由基抑制率的10.11%至73.50%。
