Random-primed cDNA synthesis facilitates the isolation of multiple 5'-cDNA ends by RACE.

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作者信息

Harvey R J, Darlison M G

机构信息

MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, UK.

出版信息

Nucleic Acids Res. 1991 Jul 25;19(14):4002. doi: 10.1093/nar/19.14.4002.

DOI:10.1093/nar/19.14.4002

PMID:1907367

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328501/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95c8/328501/af8cfa0dd6ec/nar00094-0203-a.jpg

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本文引用的文献

Direct clone characterization from plaques and colonies by the polymerase chain reaction.通过聚合酶链反应对噬菌斑和菌落进行直接克隆鉴定。

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Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.从稀有转录本快速生成全长cDNA：使用单一基因特异性寡核苷酸引物进行扩增

Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002. doi: 10.1073/pnas.85.23.8998.

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