乙酰脒化猪心乳酸脱氢酶的热稳定性、碱性稳定性和胰蛋白酶稳定性增强。
Enhanced heat, alkaline and tryptic stability of acetamidinated pig heart lactate dehydrogenase.
作者信息
Tuengler P, Pfleiderer G
出版信息
Biochim Biophys Acta. 1977 Sep 15;484(1):1-8. doi: 10.1016/0005-2744(77)90108-5.
PMID:19075
Abstract
Modification of 17 from 24 lysine residues in pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with methyl aceimidate yields an enzyme derivative with enhanced stability toward meat and alkaline denaturation as well as tryptic digestion. The specific activity of the modified enzyme is only slightly reduced
摘要
用乙酰亚胺甲酯修饰猪心乳酸脱氢酶（L-乳酸：NAD⁺氧化还原酶，EC 1.1.1.27）的24个赖氨酸残基中的17个，产生一种对热、碱性变性以及胰蛋白酶消化具有增强稳定性的酶衍生物。修饰后酶的比活性仅略有降低。
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