Step-wise cross-linking of pig heart lactate dehydrogenase by a heterobifunctional reagent.
作者信息
Kolkenbrock H, Kiltz H H, Trommer W E
出版信息
Biochim Biophys Acta. 1978 Aug 21;535(2):216-21. doi: 10.1016/0005-2795(78)90087-9.
PMID:567067
Abstract
Cross-linking of the essential cysteine-165 of pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with lysine-179 was achieved by stepwise reaction with N-(4-azidocarbonyl-3-hydroxy-phenyl)-maleimide. These two residues are connected by kind of a hydrophobic channel which suggests that the essential cysteine is not modified via the active center as previously assumed.
摘要
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