Inhibition of thymidylate synthase by glyceraldehyde 3-phosphate.
作者信息
Bures A K, Daron H H, Aull J L
机构信息
Department of Chemistry, Alabama Agricultural Experiment Station, Auburn University 36849-5312.
出版信息
Int J Biochem. 1991;23(7-8):733-6. doi: 10.1016/0020-711x(91)90045-o.
PMID:1907578
Abstract
- A number of common metabolites which had carbonyl and/or phosphate groups were tested for their ability to alter the activity of thymidylate synthase from Lactobacillus casei. Glyceraldehyde 3-phosphate was found to be an effective inhibitor of thymidylate synthase. 2. Glyceraldehyde 3-phosphate reversibly inhibited thymidylate synthase with a K1 of 12-13 microM; the inhibition was competitive with dUMP and noncompetitive with 5,10-methylenetetrahydrofolate which is consistent with an ordered addition of substrates.
摘要
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