Sweeny D J, Barnes S, Diasio R B
Division of Clinical Pharmacology, University of Alabama, Birmingham 35294.
JPEN J Parenter Enteral Nutr. 1991 May-Jun;15(3):303-6. doi: 10.1177/0148607191015003303.
The influence of the taurine-containing amino acid mixture Trophamine on the pattern of bile acid conjugation was examined in the isolated perfused rat liver using cholic acid as the bile acid substrate. In all experiments, greater than 97% of the cholic acid appearing in bile was conjugated with taurine or glycine. The pattern of taurine and glycine bile acid conjugation, however, was dependent on the availability of taurine in the perfusate medium. Thus, in the absence of Trophamine infusion, the percentage of cholic acid conjugated with taurine (ie, taurocholate) declined throughout the course of the cholic acid infusion. Trophamine infusion increased the ratio of biliary taurocholate/glycocholate by 4.5-fold over that observed in the absence of the amino acid infusion. Increasing the amount of taurine in Trophamine by 2- or 5-fold resulted in a 1.8- and 4.3-fold increase, respectively, in the taurocholate/glycocholate ratio over that observed during the Trophamine infusion. Infusion of taurine alone, at an equimolar concentration of taurine as that in Trophamine, resulted in a similar taurocholate/glycocholate ratio as that observed during the Trophamine infusion. These data indicate that taurine availability, even in the presence of high concentrations of glycine and other amino acids in Trophamine, appears to be the most important factor in determining the pattern of bile acid conjugation in the isolated perfused rat liver.
以胆酸作为胆汁酸底物，在离体灌注大鼠肝脏中研究了含牛磺酸的氨基酸混合物Trophamine对胆汁酸结合模式的影响。在所有实验中，胆汁中出现的胆酸超过97%与牛磺酸或甘氨酸结合。然而，牛磺酸和甘氨酸胆汁酸结合的模式取决于灌注液培养基中牛磺酸的可用性。因此，在不输注Trophamine的情况下，在胆酸输注过程中，与牛磺酸结合的胆酸（即牛磺胆酸盐）百分比下降。输注Trophamine使胆汁中牛磺胆酸盐/甘氨胆酸盐的比例比未输注氨基酸时观察到的增加了4.5倍。将Trophamine中牛磺酸的量增加2倍或5倍，导致牛磺胆酸盐/甘氨胆酸盐的比例分别比输注Trophamine时观察到的增加1.8倍和4.3倍。以与Trophamine中等摩尔浓度的牛磺酸单独输注，导致牛磺胆酸盐/甘氨胆酸盐的比例与输注Trophamine时观察到的相似。这些数据表明，即使在Trophamine中存在高浓度的甘氨酸和其他氨基酸的情况下，牛磺酸的可用性似乎也是决定离体灌注大鼠肝脏中胆汁酸结合模式的最重要因素。
