Kwon Sun Jae, Jin Hak Chul, Lee Soohyun, Nam Myung Hee, Chung Joo Hee, Kwon Soon Il, Ryu Choong-Min, Park Ohkmae K
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Plant J. 2009 Apr;58(2):235-45. doi: 10.1111/j.1365-313X.2008.03772.x. Epub 2008 Dec 10.
Systemic resistance is induced by necrotizing pathogenic microbes and non-pathogenic rhizobacteria and confers protection against a broad range of pathogens. Here we show that Arabidopsis GDSL LIPASE-LIKE 1 (GLIP1) plays an important role in plant immunity, eliciting both local and systemic resistance in plants. GLIP1 functions independently of salicylic acid but requires ethylene signaling. Enhancement of GLIP1 expression in plants increases resistance to pathogens including Alternaria brassicicola, Erwinia carotovora and Pseudomonas syringae, and limits their growth at the infection site. Furthermore, local treatment with GLIP1 proteins is sufficient for the activation of systemic resistance, inducing both resistance gene expression and pathogen resistance in systemic leaves. The PDF1.2-inducing activity accumulates in petiole exudates in a GLIP1-dependent manner and is fractionated in the size range of less than 10 kDa as determined by size exclusion chromatography. Our results demonstrate that GLIP1-elicited systemic resistance is dependent on ethylene signaling and provide evidence that GLIP1 may mediate the production of a systemic signaling molecule(s).
坏死性致病微生物和非致病性根际细菌可诱导植物产生系统抗性，从而对多种病原体提供保护。在此，我们表明拟南芥GDSL类脂肪酶1（GLIP1）在植物免疫中发挥重要作用，可引发植物的局部和系统抗性。GLIP1的功能独立于水杨酸，但需要乙烯信号传导。增强植物中GLIP1的表达可增加对包括链格孢、胡萝卜软腐欧文氏菌和丁香假单胞菌在内的病原体的抗性，并限制它们在感染部位的生长。此外，用GLIP1蛋白进行局部处理足以激活系统抗性，诱导系统叶片中的抗性基因表达和病原体抗性。PDF1.2诱导活性以GLIP1依赖的方式在叶柄渗出物中积累，通过尺寸排阻色谱法测定，其分子量小于10 kDa。我们的结果表明，GLIP1引发的系统抗性依赖于乙烯信号传导，并提供了GLIP1可能介导一种或多种系统信号分子产生的证据。
