单萜类化合物对奇异变形杆菌群体感应分化及溶血素活性的影响。
The effect of monoterpenes on swarming differentiation and haemolysin activity in Proteus mirabilis.
作者信息
Echeverrigaray Sergio, Michelim Lessandra, Longaray Delamare Ana Paula, Andrade Cristiane Paim, Pinto da Costa Sérgio Olavo, Zacaria Jucimar
机构信息
Research Laboratory of Microbiology, Institute of Biotechnology, University of Caxias do Sul, Rua Francisco Getúlio Vargas, 1130, Caxias do Sul, Brazil 95070-560.
出版信息
Molecules. 2008 Dec 15;13(12):3107-16. doi: 10.3390/molecules13123107.
Urinary tract infection by Proteus mirabilis depends on several virulence properties that are coordinately regulated with swarming differentiation. Here we report the antibacterial and anti-swarming effect of seventeen terpenoids, and the effect of subinhibitory concentrations of five selected terpenoids on swarming, biofilm formation and haemolysin activity. The results showed that all the terpenes evaluated, particularly oxygenated terpenoids, inhibited P. mirabilis with MIC values ranging between 3 and 10 mg/L. Moreover, citral, citronellol and geraniol effectively inhibit P. mirabilis swarming in a dose dependent manner, reducing swimming/swarming cell differentiation and haemolysin activity at 1/10 MIC concentration. The inhibition of P. mirabilis swarming and virulence factor expression by selected oxygenated terpenoids suggest that essential oils with high concentration of these compounds have the potential to be developed as products for preventing P. mirabilis infections.
奇异变形杆菌引起的尿路感染取决于多种毒力特性，这些特性与群体感应分化协同调控。在此，我们报告了17种萜类化合物的抗菌和抗群体感应作用，以及5种选定萜类化合物的亚抑菌浓度对群体感应、生物膜形成和溶血素活性的影响。结果表明，所有评估的萜类化合物，尤其是氧化萜类化合物，均能抑制奇异变形杆菌，其最低抑菌浓度（MIC）值在3至10mg/L之间。此外，柠檬醛、香茅醇和香叶醇以剂量依赖方式有效抑制奇异变形杆菌群体感应，在1/10 MIC浓度下降低游动/群体感应细胞分化和溶血素活性。选定的氧化萜类化合物对奇异变形杆菌群体感应和毒力因子表达的抑制作用表明，含有高浓度这些化合物的精油有潜力开发成为预防奇异变形杆菌感染的产品。
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