在猪心肌梗死模型中评估CD151改善心肌血液灌注的情况。
Assessment of myocardial blood perfusion improved by CD151 in a pig myocardial infarction model.
作者信息
Zuo Hou-juan, Liu Zheng-xiang, Liu Xiao-chun, Yang Jun, Liu Tao, Wen Sha, Wang Dao-wen, Zhang Xin
机构信息
Department of Cardiology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Acta Pharmacol Sin. 2009 Jan;30(1):70-7. doi: 10.1038/aps.2008.10. Epub 2008 Dec 15.
Abstract
AIM
To appraise the efficacy of CD151-induced myocardial therapeutic angiogenesis in a pig myocardial infarction model.
METHODS
CD151 and anti-CD151 were constructed into the recombinant adeno-associated virus (rAAV) vector. All 26 pigs were subjected to coronary artery ligation or no surgery. Eight weeks after coronary artery ligation, the expression of CD151 was measured by Western blot and immunostaining. Capillary density was evaluated using immunostaining for von Willebrand factor (vWF). 13N-labeled NH3 positron emission computed tomography ([13N]NH3PET) was measured to assess regional myocardial perfusion and the defect area.
RESULTS
CD151 gene delivery could increase the expression of CD151 at protein level. Over-expression of CD151 increased the density of total capillaries in the ischemic myocardium, significantly improved the blood perfusion and reduced the defect area percentage.
CONCLUSION
This study demonstrated that the rAAV-mediated CD151 gene delivery promoted efficient neovascularization and increased the blood perfusion after myocardial infarction in pigs.
摘要
目的
评估CD151诱导猪心肌梗死模型心肌治疗性血管生成的疗效。
方法
将CD151和抗CD151构建到重组腺相关病毒（rAAV）载体中。26只猪全部接受冠状动脉结扎或不进行手术。冠状动脉结扎8周后，通过蛋白质印迹法和免疫染色检测CD151的表达。使用抗血管性血友病因子（vWF）免疫染色评估毛细血管密度。测量13N标记的NH3正电子发射计算机断层扫描（[13N]NH3PET）以评估局部心肌灌注和缺损面积。
结果
CD151基因递送可在蛋白质水平上增加CD151的表达。CD151的过表达增加了缺血心肌中总毛细血管的密度，显著改善了血液灌注并降低了缺损面积百分比。
结论
本研究表明，rAAV介导的CD151基因递送促进了猪心肌梗死后有效的新生血管形成并增加了血液灌注。
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