Santiago Ludmilla Nadir, de Camargo Fenley Juliana, Braga Lúcia Campanario, Cordeiro José Antônio, Cury Patrícia M
Department of Pathology and Forensic Medicine, Laboratory of Experimental Pathology, Departament of Pathology and Forensic Medicine, Faculdade de Medicina de São José do Rio Preto, FAMERP, Brazil.
Int J Clin Exp Pathol. 2009;2(2):176-81. Epub 2008 Aug 25.
Few studies have used Balb/c mice as an animal model for lung carcinogenesis. In this study, we investigated the effect of different doses of cigarette smoking in the urethane-induced Balb/c mouse lung cancer model. After injection of 3mg/kg urethane intraperitoneally, the mice were then exposed to tobacco smoke once or twice a day, five times a week, in a closed chamber. The animals were randomly divided into four groups. The control group (G0) received urethane only. The experimental groups (G1, G2 and G3) received urethane and exposure to the smoke of 3 cigarettes for 10 minutes once a day, 3 cigarettes for 10 minutes twice a day, and 6 cigarettes for 10 minutes twice a day, respectively. The mice were sacrificed after 16 weeks of exposure, and the number of nodules and hyperplasia in the lungs was counted. The results showed no statistically significant difference in the mean number of nodules and hyperplasia among the different groups, suggesting that the Balb/c mice are not suitable to study the pathogenesis of tobacco smoking-induced tumor progression in the lungs.
很少有研究将Balb/c小鼠用作肺癌发生的动物模型。在本研究中，我们在氨基甲酸乙酯诱导的Balb/c小鼠肺癌模型中研究了不同剂量吸烟的影响。腹腔注射3mg/kg氨基甲酸乙酯后，将小鼠置于封闭舱内，每周5天，每天暴露于烟草烟雾1次或2次。动物被随机分为四组。对照组（G0）仅接受氨基甲酸乙酯。实验组（G1、G2和G3）分别接受氨基甲酸乙酯并每天暴露于3支香烟的烟雾中10分钟、每天暴露于3支香烟的烟雾中2次，每次10分钟、以及每天暴露于6支香烟的烟雾中2次，每次10分钟。暴露16周后处死小鼠，计数肺内结节和增生的数量。结果显示不同组之间结节和增生的平均数量无统计学显著差异，表明Balb/c小鼠不适合用于研究吸烟诱导的肺部肿瘤进展的发病机制。
