对来自HIV患者的福尔马林固定石蜡包埋口腔HPV病变进行定量蛋白质组学分析。
Quantitative Proteomic Analysis of Formalin Fixed Paraffin Embedded Oral HPV Lesions from HIV Patients.
作者信息
Jain Mohit Raja, Liu Tong, Hu Jun, Darfler Marlene, Fitzhugh Valerie, Rinaggio Joseph, Li Hong
机构信息
Center for Advanced Proteomics Research and Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School Cancer Center, 205 South Orange Avenue, Newark, NJ 07103.
出版信息
Open Proteomics J. 2008;1:40-45. doi: 10.2174/1875039700801010040.
Human immunodeficiency virus (HIV) infection is associated with dysplastic changes in oral human papilloma virus (HPV) lesions, suggesting changes in keratinocytes. In the present study, we seek to identify proteomic changes in oral HPV lesions between HIV(+) and HIV(-) patients. While fresh tissues represent the most desirable samples for proteomic investigations, they are often difficult to obtain in large numbers under clinical settings. We therefore have developed a new method to identify protein changes in formalin fixed and paraffin-embedded (FFPE) oral HPV lesions utilizing iTRAQ™ technology in conjunction with Liquid Tissue® sample preparation method. Using this method, we identified nine proteins that were differentially expressed in oral HPV lesions as a result of HIV infection. The quantitative proteomic method presented here will be valuable for others who plan to analyze FFPE tissues.
人类免疫缺陷病毒（HIV）感染与口腔人乳头瘤病毒（HPV）病变的发育异常变化相关，提示角质形成细胞发生了改变。在本研究中，我们试图确定HIV阳性和HIV阴性患者口腔HPV病变中的蛋白质组变化。虽然新鲜组织是蛋白质组学研究最理想的样本，但在临床环境下往往难以大量获取。因此，我们开发了一种新方法，利用iTRAQ™技术结合Liquid Tissue®样本制备方法，来鉴定福尔马林固定石蜡包埋（FFPE）口腔HPV病变中的蛋白质变化。使用这种方法，我们鉴定出了9种因HIV感染而在口腔HPV病变中差异表达的蛋白质。本文介绍的定量蛋白质组学方法对其他计划分析FFPE组织的人将具有重要价值。
Zotero 插件