口腔福尔马林固定石蜡包埋鳞状乳头状瘤中人乳头瘤病毒DNA的检测
Detection of human papillomavirus DNA in formalin-fixed, paraffin-embedded squamous papillomas of the oral cavity.
作者信息
Daigrepont Jack, Cameron Jennifer E, Wright Kelly L, Cordell Kitrina G, Rosebush Molly S
机构信息
DDS, Staff Dentist, SWLA Center for Health Services, Lake Charles, LA, USA.
PhD, Assistant Professor of Microbiology, Immunology & Parasitology, Louisiana State University, New Orleans, LA, USA.
出版信息
J Clin Exp Dent. 2018 Oct 1;10(10):e979-e983. doi: 10.4317/jced.55187. eCollection 2018 Oct.
BACKGROUND
Squamous papillomas are exophytic proliferations of surface oral epithelium. Human papillomavirus (HPV) infection is widely accepted as the etiology of squamous papillomas however the virus cannot be detected in a significant percentage of lesions.
MATERIAL AND METHODS
Using polymerase chain reaction (PCR), we tested 35 formalin-fixed paraffin-embedded (FFPE) squamous papillomas for the presence of HPV DNA.
RESULTS
Six papillomas (17%) tested positive for HPV DNA; four contained HPV-6 and two contained HPV-11. Given that β-globin DNA was only identified in half of the samples, DNA degradation appears to have significantly impacted the results.
CONCLUSIONS
The results likely represent an underestimation of the true number of HPV-positive specimens in our study. Potential explanations for HPV-negative squamous papillomas include transient HPV infection, failure of the experiment to detect HPV if present, or the possibility that some lesions may not result from HPV infection. HPV, PCR, FFPE, papilloma, oral.
背景
鳞状乳头状瘤是口腔表面上皮的外生性增生。人乳头瘤病毒（HPV）感染被广泛认为是鳞状乳头状瘤的病因，然而在相当比例的病变中无法检测到该病毒。
材料与方法
我们使用聚合酶链反应（PCR）检测了35例福尔马林固定石蜡包埋（FFPE）的鳞状乳头状瘤中HPV DNA的存在情况。
结果
6例乳头状瘤（17%）HPV DNA检测呈阳性；4例含有HPV - 6，2例含有HPV - 11。鉴于仅在一半的样本中鉴定出β - 珠蛋白DNA，DNA降解似乎对结果产生了显著影响。
结论
我们研究的结果可能低估了HPV阳性标本的实际数量。HPV阴性鳞状乳头状瘤的潜在解释包括HPV的短暂感染、实验未能检测到存在的HPV，或者某些病变可能并非由HPV感染引起。HPV、PCR、FFPE、乳头状瘤、口腔
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