Stokes Robert J, McKenzie Fiona, McFarlane Emma, Ricketts Alastair, Tetley Laurence, Faulds Karen, Alexander James, Graham Duncan
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UKG1 1XL.
Analyst. 2009 Jan;134(1):170-5. doi: 10.1039/b815117b. Epub 2008 Nov 19.
Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
在固定和染色之前用金和银纳米颗粒处理过的骨髓源性免疫细胞（巨噬细胞），已通过多波长线扫描表面增强共振拉曼散射（SERRS）映射进行了分析。该方法在短分析时间内具有高选择性和灵敏度，可识别次级溶酶体中的纳米颗粒聚集体。使用常规细胞染色剂，在四个激发波长下对荧光、拉曼和SERRS的输出进行定量，证明了在更长的生物相容性波长下使用纳米颗粒与细胞染色剂进行卓越细胞映射的潜力。
