Department of Orthopaedics, Affiliated Mental Health Center of Nantong University, Nantong, People's Republic of China.
Cell Mol Neurobiol. 2013 Mar;33(2):213-21. doi: 10.1007/s10571-012-9887-2. Epub 2012 Nov 6.
As a novel cell cycle protein, Spy1 enhances cell proliferation, promotes the G1/S transition as well as inhibits apoptosis in response to UV irradiation. Spy1 levels are tightly regulated during mammary development, and overexpression of Spy1 accelerates tumorigenesis in vivo. But little is known about the role of Spy1 in the pathological process of damage and regeneration of the peripheral nervous system. Here we established a rat sciatic nerve crush (SNC) model to examine the spatiotemporal expression of Spy1. Spy1 expression was elevated gradually after sciatic nerve crush and peaked at day 3. The alteration was due to the increased expression of Spy1 in axons and Schwann cells after SNC. Spy1 expression correlated closely with Schwann cells proliferation in sciatic nerve post injury. Furthermore, Spy1 largely localized in axons in the crushed segment, but rarely co-localized with GAP43. These findings suggested that Spy1 participated in the pathological process response to sciatic nerve injury and may be associated with Schwann cells proliferation and axons regeneration.
作为一种新型细胞周期蛋白，Spy1 可增强细胞增殖，促进 G1/S 期过渡，并抑制紫外线照射引起的细胞凋亡。Spy1 在乳腺发育过程中受到严格调控，过表达 Spy1 可加速体内肿瘤的发生。但是，关于 Spy1 在周围神经系统损伤和再生的病理过程中的作用知之甚少。在这里，我们建立了大鼠坐骨神经挤压（SNC）模型，以检查 Spy1 的时空表达。坐骨神经挤压后 Spy1 的表达逐渐升高，并在第 3 天达到峰值。这种变化是由于 SNC 后 Spy1 在轴突和施万细胞中的表达增加所致。Spy1 的表达与损伤后坐骨神经中施万细胞的增殖密切相关。此外，Spy1 主要定位于挤压段的轴突中，但很少与 GAP43 共定位。这些发现表明，Spy1 参与了对坐骨神经损伤的病理过程反应，可能与施万细胞增殖和轴突再生有关。
