QU Ling, LIANG Xiao-chun, ZHANG Hong, WU Qun-li, SUN Lian-qing, GU Bei
Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing (100730), China.
Chin J Integr Med. 2008 Dec;14(4):293-7. doi: 10.1007/s11655-008-0293-z. Epub 2008 Dec 12.
To investigate the effect of Jinmaitong (JMT) serum on the proliferation of rat Schwann cells (SCs) primarily cultured in high glucose medium.
SCs were primarily cultured in Dulbecco's minmum essential medium (DMEM control), 50 mmol/L glucose medium (50 mmol/L Glu), 75 mmol/L glucose medium (75 mmol/L Glu), as well as 50 mmol/L glucose medium, with different concentrations of JMT serum (undiluted, 1:2 diluted and 1:8 diluted) and Neurotropin (Ntp), respectively. The proliferation of SCs under different conditions was detected by MTT.
SCs grew exuberantly in DMEM within 24-72 h, but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48, 72 and 96 h, which showed that both were significantly different compared to the control group (P<0.01). The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu (P<0.05). Spearman's rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose (r=-0.471, P<0.01). Excluding the time factor, partial correlation showed similar results (r=-0.679, P<0.01). After 48 h, the proliferation of SCs increased significantly in JMT1:2 and Ntp compared with 50 mmol/L Glu (control 0.437+or-0.019, 50 mmol/L Glu 0.367+or-0.035, JMT1:2 0.426+or-0.024, Ntp 0.422+or-0.013; P<0.01), and there were no statistically significant differences among the JMT groups, the Ntp group and the control group (P>0.05).
The proliferation of SCs was inhibited in high glucose medium, and the inhibition was reduced by different concentrations of JMT serum, especially at JMT1:2.
探讨金马通（JMT）含药血清对高糖培养基中大鼠雪旺细胞（SCs）增殖的影响。
将SCs分别培养于杜氏改良 Eagle 培养基（DMEM 对照组）、50 mmol/L 葡萄糖培养基（50 mmol/L Glu）、75 mmol/L 葡萄糖培养基（75 mmol/L Glu）以及添加不同浓度 JMT 含药血清（未稀释、1:2 稀释和 1:8 稀释）和神经妥乐平（Ntp）的 50 mmol/L 葡萄糖培养基中。采用 MTT 法检测不同条件下 SCs 的增殖情况。
SCs 在 DMEM 中培养 24 - 72 h 生长旺盛，但在 96 h 时生长减缓。在 50 mmol/L Glu 和 75 mmol/L Glu 中培养 48、72 和 96 h 后，SCs 的增殖受到抑制，与对照组相比差异有统计学意义（P<0.01）。75 mmol/L Glu 组的抑制作用比 50 mmol/L Glu 组更显著（P<0.05）。Spearman 秩相关分析显示，SCs 的增殖与葡萄糖浓度呈负相关（r = -0.471，P<0.01）。排除时间因素后，偏相关分析结果相似（r = -0.679，P<0.01）。48 h 后，与 50 mmol/L Glu 组相比，JMT1:2 组和 Ntp 组中 SCs 的增殖显著增加（对照组 0.437±0.019，50 mmol/L Glu 组 0.367±0.035，JMT1:2 组 0.426±0.024，Ntp 组 0.422±0.013；P<0.01），JMT 各浓度组、Ntp 组与对照组之间差异无统计学意义（P>0.05）。
高糖培养基可抑制SCs 的增殖，不同浓度的 JMT 含药血清可减轻这种抑制作用，尤其是 JMT1:2 浓度组。
