Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China.
Department of Endocrinology, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China.
Neural Regen Res. 2012 Oct 25;7(30):2340-6. doi: 10.3969/j.issn.1673-5374.2012.30.003.
Cultured Schwann cells were treated with 5.6 mM and 50 mM glucose alternating every 8 hours to simulate intermittent high glucose. The present study analyzed the neuroprotective effects of 1, 10 and 100 μM ginsenoside Rb1 on oxidative damage and apoptosis in Schwann cells induced by intermittent high glucose. Flow cytometry demonstrated that ginsenoside Rb1 reduced intermittent high glucose-mediated reactive oxygen species production. Enzyme linked immunosorbent assay showed that 8-hydroxy-2-deoxy guanosine levels in Schwann cells decreased following ginsenoside Rb1 treatment. Quantitative real-time reverse transcription-PCR and western blot assay results revealed that ginsenoside Rb1 inhibited intermittent high glucose-upregulated Bax expression, but antagonized intermittent high glucose-downregulated Bcl-2 expression in Schwann cells. These effects were most pronounced with 100 μM ginsenoside Rb1. These results indicate that ginsenoside Rb1 inhibits intermittent high glucose-induced oxidative stress and apoptosis in Schwann cells.
将培养的施万细胞用 5.6mM 和 50mM 葡萄糖交替处理，每 8 小时处理一次，以模拟间歇性高葡萄糖。本研究分析了 1、10 和 100μM 人参皂苷 Rb1 对间歇性高葡萄糖诱导的施万细胞氧化损伤和细胞凋亡的神经保护作用。流式细胞术表明，人参皂苷 Rb1 减少了间歇性高葡萄糖介导的活性氧的产生。酶联免疫吸附试验显示，施万细胞中 8-羟基-2-脱氧鸟苷水平在人参皂苷 Rb1 处理后降低。实时定量逆转录聚合酶链反应和 Western blot 检测结果表明，人参皂苷 Rb1 抑制了间歇性高葡萄糖上调的 Bax 表达，但拮抗了间歇性高葡萄糖下调的 Bcl-2 表达在施万细胞中。100μM 人参皂苷 Rb1 的效果最为显著。这些结果表明，人参皂苷 Rb1 抑制了间歇性高葡萄糖诱导的施万细胞氧化应激和细胞凋亡。
