中国太湖水体中微囊藻毒素-LR的测定

Determination of microcystin-LR in water from Lake Tai, China.

作者信息

Xiao Fu-Gang, Zhao Xiao-Lian, Tang Jian, Gu Xiao-Hong, Zhang Jing-Ping, Niu Wei-Min

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.

出版信息

Bull Environ Contam Toxicol. 2009 Feb;82(2):230-3. doi: 10.1007/s00128-008-9623-2. Epub 2008 Dec 13.

DOI:10.1007/s00128-008-9623-2

PMID:19082909

Abstract

Microcystin-LR (MC-LR) is a heptapeptide hepatotoxin produced by cyanobacteria. Immunoaffinity chromatography (IAC) column was prepared with CNBr-activated Sepharose 4B and monoclonal antibody of MC-LR. Water sample was cleaned up by IAC column and the content of MC-LR in water was determined by liquid chromatography-mass spectrometry (LC-MS). The results suggested that the IAC column exhibited highly selective specificity for MC-LR and selective removed interference from complex water sample. Water sample was concentrated for 2,000-fold through once purification. Cyanobacterial blooms had broken out in 2007 in Lake Tai, the third largest freshwater lake in China. Water samples from two parts of Lake Tai had been analyzed. The highest concentration of MC-LR in water from Lake Tai was 0.522 microg/L.

摘要

微囊藻毒素-LR（MC-LR）是一种由蓝藻产生的七肽肝毒素。用溴化氰活化的琼脂糖凝胶4B和MC-LR单克隆抗体制备免疫亲和色谱（IAC）柱。水样通过IAC柱净化，水中MC-LR的含量通过液相色谱-质谱联用（LC-MS）测定。结果表明，IAC柱对MC-LR具有高度的选择性特异性，能选择性地去除复杂水样中的干扰物。水样经一次纯化后浓缩了2000倍。2007年中国第三大淡水湖太湖爆发了蓝藻水华。对太湖两个区域的水样进行了分析。太湖水中MC-LR的最高浓度为0.522微克/升。