石榴酸对大鼠肌肉和脂肪组织的掺入及影响
Incorporation and effects of punicic acid on muscle and adipose tissues of rats.
作者信息
de Melo Illana Louise Pereira, de Oliveira e Silva Ana Mara, de Carvalho Eliane Bonifácio Teixeira, Yoshime Luciana Tedesco, Sattler José Augusto Gasparotto, Mancini-Filho Jorge
机构信息
Department of Food and Experimental Nutrition, Laboratory of Lipids, Faculty of Pharmaceutical Sciences, São Paulo, University of São Paulo, Av. Prof. Lineu Prestes, 580 - Bloco 14, CEP: 05508-900, São Paulo, Brazil.
Federal University of Sergipe, Biological Science and Health Center, Aracaju, Sergipe, Brazil.
出版信息
Lipids Health Dis. 2016 Feb 27;15:40. doi: 10.1186/s12944-016-0214-7.
BACKGROUND
This study evaluated the effect of pomegranate seed oil (PSO) supplementation, rich in punicic acid (55 %/C18:3-9c,11 t,13c/CLNA), on the lipid profile and on the biochemical and oxidative parameters in the gastrocnemius muscle and adipose tissues of healthy rats. Linseed oil (LO), rich in linolenic acid (52 %/C18:3-9c12c15c/LNA) was used for comparison.
METHODS
Male Wistar rats (n = 56) were distributed in seven groups: control (water); LNA 1 %, 2 % and 4 % (treated with LO); CLNA 1 %, 2 % and 4 % (treated with PSO), po for 40 days. The percentages were compared to the daily feed intake. Fatty acid profile were performed by gas chromatography, antioxidant enzymes activity by spectrophotometer and the adipocytes were isolated by collagenase tissue digestion. Analysis of variance (ANOVA) was applied to check for differences between the groups (control, LNAs and CLNAs) and principal component analysis (PCA) was used to project the groups in the factor-place (PC1 vs PC2) based on the biochemical responses assessed in the study.
RESULTS
The fatty acids profile of tissues showed that the LNA percentages were higher in the animals that were fed LO. However, PA was only detected in the adipose tissues. Conjugated linoleic acid (CLA) was present in all the tissues of the animals supplemented with PSO, in a dose dependent manner, and 9c11t-CLA was the predominant isomer. Nevertheless there were no changes in the total weight gain of the animals, the weights of the tissues, and the oxidative stress parameters in the muscle. In addition, there was an increase in the size of the epididymal fat cells in the groups treated with PSO. Principal component analysis (PCA) showed that the CLNAs groups were arranged separately with a cumulative variance of 68.47 %.
CONCLUSIONS
The results show that PSO can be used as a source of CLAs but that it does not cause changes in body modulation and does not interfere in the antioxidant activity of healthy rats.
背景
本研究评估了富含石榴酸（55%/C18:3-9c,11t,13c/共轭亚油酸）的石榴籽油（PSO）补充剂对健康大鼠腓肠肌和脂肪组织脂质谱、生化及氧化参数的影响。以富含亚麻酸（52%/C18:3-9c12c15c/α-亚麻酸）的亚麻籽油（LO）作为对照。
方法
将56只雄性Wistar大鼠分为七组：对照组（给予水）；1%、2%和4%α-亚麻酸组（给予LO处理）；1%、2%和4%共轭亚油酸组（给予PSO处理），经口给药40天。这些百分比是相对于每日饲料摄入量而言的。通过气相色谱法分析脂肪酸谱，用分光光度计测定抗氧化酶活性，通过胶原酶组织消化法分离脂肪细胞。应用方差分析（ANOVA）来检验各组（对照组、α-亚麻酸组和共轭亚油酸组）之间的差异，并基于本研究评估的生化反应，使用主成分分析（PCA）将各组投影到因子空间（PC1与PC2）中。
结果
组织的脂肪酸谱显示，喂食LO的动物体内α-亚麻酸百分比更高。然而，仅在脂肪组织中检测到石榴酸。共轭亚油酸（CLA）以剂量依赖性方式存在于补充PSO的动物的所有组织中，且9c11t-CLA是主要异构体。然而，动物的总体重增加、组织重量以及肌肉中的氧化应激参数均无变化。此外，PSO处理组的附睾脂肪细胞大小增加。主成分分析（PCA）表明，共轭亚油酸组单独排列，累积方差为68.47%。
结论
结果表明，PSO可作为CLA的来源，但它不会引起身体调节的变化，也不会干扰健康大鼠的抗氧化活性。
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