Fondevila M, Phillips C, Naveran N, Fernandez L, Cerezo M, Salas A, Carracedo A, Lareu M V
Institute of Legal Medicine, Genomic Medicine Group, Universidade de Santiago de Compostela, Santiago de Compostela, Galicia, Spain.
Forensic Sci Int Genet. 2008 Jun;2(3):212-8. doi: 10.1016/j.fsigen.2008.02.005. Epub 2008 Apr 10.
Applying two extraction protocols to isolate DNA from a charred femur recovered after a major forest fire, a range of established and recently developed forensic marker sets that included mini-STRs and SNPs were used to type the sample and confirm identity by comparison to a claimed daughter of the deceased. Identification of the remains suggested that the individual had been dead for 10 years and the DNA was therefore likely to be severely degraded from the combined effects of decomposition and exposure to very high temperatures. We used new marker sets specifically developed to analyze degraded DNA comprising both reduced-length amplicon STR sets and autosomal SNP multiplexes, giving an opportunity to assess the ability of each approach to successfully type highly degraded material from a challenging case. The results also suggest a modified ancient DNA extraction procedure offers improved typing success from degraded skeletal material.
应用两种提取方案从一场重大森林火灾后找到的烧焦股骨中分离DNA，使用了一系列既定的和最近开发的法医标记集，包括微型短串联重复序列（mini-STRs）和单核苷酸多态性（SNPs），对样本进行分型，并通过与声称是死者女儿的人进行比对来确认身份。遗骸鉴定表明该个体已死亡10年，因此由于分解和暴露于非常高的温度的综合影响，DNA可能已严重降解。我们使用了专门为分析降解DNA而开发的新标记集，包括缩短长度的扩增子STR集和常染色体SNP多重检测，从而有机会评估每种方法成功对一个具有挑战性案例中的高度降解材料进行分型的能力。结果还表明，一种改良的古代DNA提取程序能提高从降解骨骼材料中获得分型成功的几率。
