Mathai A, Radhakrishnan V V, Thomas M
Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, India.
Eur J Clin Microbiol Infect Dis. 1991 May;10(5):440-3. doi: 10.1007/BF01968025.
A simple dot enzyme immunoassay (Dot-EIA) was carried out to detect antibody to Mycobacterium tuberculosis antigen 5 in cerebrospinal fluid (CSF) specimens from 40 patients with a clinical diagnosis of tuberculous meningitis (TBM). The assay gave a positive reaction in all ten patients with culture proven TBM. In 30 culture negative patients with TBM, the assay was positive at a titre of 1:16 in 18 patients. In 40 patients with non-tuberculous neurological diseases (control group) the assay was negative at a titre of 1:16. The Dot-EIA had an overall sensitivity of 70% and a specificity of 100% in the diagnosis of TBM. This assay could be used as a rapid screening test to establish the diagnosis of TBM, particularly in patients in whom bacteriological investigations for Mycobacterium tuberculosis in CSF specimens are negative.
采用简单的斑点酶免疫测定法（Dot-EIA）检测40例临床诊断为结核性脑膜炎（TBM）患者脑脊液（CSF）标本中结核分枝杆菌抗原5抗体。在所有10例经培养证实为TBM的患者中，该检测呈阳性反应。在30例培养阴性的TBM患者中，18例患者检测在滴度为1:16时呈阳性。在40例非结核性神经疾病患者（对照组）中，检测在滴度为1:16时呈阴性。Dot-EIA在TBM诊断中的总体敏感性为70%，特异性为100%。该检测可作为快速筛查试验用于确立TBM诊断，特别是对于CSF标本中结核分枝杆菌细菌学检查阴性的患者。
