在酶联免疫吸附测定中，对含有麻疹、弓形虫和γ球蛋白抗原的各种塑料微量滴定板进行评估。
Evaluation of various plastic microtiter plates with measles, toxoplasma, and gamma globulin antigens in enzyme-linked immunosorbent assays.
作者信息
Shekarchi I C, Sever J L, Lee Y J, Castellano G, Madden D L
出版信息
J Clin Microbiol. 1984 Feb;19(2):89-96. doi: 10.1128/jcm.19.2.89-96.1984.
Abstract
Seventeen lots of microtiter plates which differed in lot, batch, plastic type, or manufacturer were evaluated as solid-phase carriers in enzyme-linked immunosorbent assays for antibodies to measles, toxoplasma, and human gamma globulin. Most plates of polystyrene or polyvinyl chloride were found to give acceptable binding. The final choice depended on the antigen to be attached. Variations in binding between lots, batches, and types of plastic were found. Well-to-well variation was found to be of greater statistical significance than edge effect and should be a consideration in selection of a plate lot for enzyme-linked immunosorbent assays. Lots of plates should be pretested by the investigator to determine whether there is good binding of the antigen to be used and whether there is low plate-to-plate and well-to-well variation.
摘要
评估了17批在批次、批量、塑料类型或制造商方面存在差异的微量滴定板，将其作为酶联免疫吸附测定中麻疹抗体、弓形虫抗体和人γ球蛋白的固相载体。发现大多数聚苯乙烯或聚氯乙烯板具有可接受的结合能力。最终选择取决于要连接的抗原。发现不同批次、批量和塑料类型之间的结合存在差异。发现孔间差异比边缘效应具有更大的统计学意义，在选择用于酶联免疫吸附测定的板批时应予以考虑。研究人员应预先测试板批，以确定所用抗原是否具有良好的结合能力，以及板间和孔间的差异是否较小。
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