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免疫吸附剂在分枝杆菌抗原纯化中的应用。

The use of immunoabsorbents for the purification of mycobacterial antigens.

作者信息

Daniel T M, Anderson P A

出版信息

J Lab Clin Med. 1977 Aug;90(2):354-60.

PMID:69672
Abstract

Affinity chromatography using immunoabsorbents prepared from goat antisera has been applied to the purificaton of antigens from culture filtrates of Mycobacterium tuberculosis. The optimum conditions for the preparation and use of these immunoabsorbents have been determined. Urea has been studied as an eluant, and it has been found that antigen can be eluted with 4M urea at pH 9 with satisfactory yields, minimal loss of antigenicity, and minimal disruption of the immunoabsorbent.

摘要

使用从山羊抗血清制备的免疫吸附剂进行亲和层析，已应用于从结核分枝杆菌培养滤液中纯化抗原。已确定了这些免疫吸附剂制备和使用的最佳条件。对尿素作为洗脱剂进行了研究，发现用pH 9的4M尿素可洗脱抗原，产率令人满意，抗原性损失最小，免疫吸附剂的破坏也最小。

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1
The use of immunoabsorbents for the purification of mycobacterial antigens.免疫吸附剂在分枝杆菌抗原纯化中的应用。
J Lab Clin Med. 1977 Aug;90(2):354-60.
2
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引用本文的文献

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J Clin Invest. 1981 Jul;68(1):153-62. doi: 10.1172/jci110231.
2
A longitudinal study of antibody titres to Antigen 6 in patients with bone and joint tuberculosis.骨与关节结核患者对抗原6抗体滴度的纵向研究。
Int Orthop. 1983;7(3):205-8. doi: 10.1007/BF00269507.
3
Immunological evaluation of a component isolated from Mycobacterium bovis BCG with a monoclonal antibody to M. bovis BCG.
用抗牛分枝杆菌卡介苗单克隆抗体对从牛分枝杆菌卡介苗中分离出的一种成分进行免疫学评估。
Infect Immun. 1984 Nov;46(2):519-25. doi: 10.1128/iai.46.2.519-525.1984.
4
Specificity of Mycobacterium tuberculosis antigen 5 determined with mouse monoclonal antibodies.用小鼠单克隆抗体测定结核分枝杆菌抗原5的特异性。
Infect Immun. 1984 Jul;45(1):52-5. doi: 10.1128/iai.45.1.52-55.1984.
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Network interactions in Schistosoma japonicum infection. Identification and characterization of a serologically distinct immunoregulatory auto-antiidiotypic antibody population.日本血吸虫感染中的网络相互作用。血清学上不同的免疫调节自身抗独特型抗体群体的鉴定与特征分析。
J Clin Invest. 1985 Dec;76(6):2338-47. doi: 10.1172/JCI112245.
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J Clin Microbiol. 1987 Mar;25(3):471-5. doi: 10.1128/jcm.25.3.471-475.1987.
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Demonstration of a shared epitope among mycobacterial antigens using a monoclonal antibody.使用单克隆抗体证明分枝杆菌抗原间的共同表位
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