Metzger Zvi, Blasbalg Jaron, Dotan Miri, Tsesis Igor, Weiss Ervin I
Department of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
J Endod. 2009 Jan;35(1):50-4. doi: 10.1016/j.joen.2008.09.016. Epub 2008 Nov 4.
Coaggregation is a key mechanism in biofilm formation. The aim of this study was to characterize the coaggregation of Fusobacterium nucleatum PK1594 with six Porphyromonas gingivalis strains in terms of kinetics and sugars inhibition. This coaggregation was quantitatively characterized by using a kinetic coaggregation assay. Sugar inhibition profiles were also quantitatively defined. Four types of interactions among these coaggregation partners were found: (1) fast coaggregation that was substantially inhibited by galactose, lactose, and fucose (strain PK1924); (2) fast coaggregation that was not inhibited by any of the sugars tested (strain 274); (3) slow coaggregation that was either substantially or partially inhibited by the sugars mentioned (strains HG405 and W50, respectively); and (4) strains that did not coaggregate with the fusobacteria (ATCC33277 and A7436). These results suggest that adhesin(s) other than the well-known galactose-mediated ones may be involved in coaggregation between F. nucleatum PK1594 and P. gingivalis strains.
共聚是生物膜形成中的关键机制。本研究的目的是从动力学和糖类抑制方面，表征具核梭杆菌PK1594与六种牙龈卟啉单胞菌菌株的共聚情况。通过动力学共聚试验对这种共聚进行了定量表征。还定量定义了糖类抑制谱。发现这些共聚伙伴之间存在四种类型的相互作用：（1）快速共聚，被半乳糖、乳糖和岩藻糖显著抑制（PK1924菌株）；（2）快速共聚，不受任何测试糖类抑制（274菌株）；（3）缓慢共聚，分别被上述糖类显著或部分抑制（HG405和W50菌株）；（4）不与梭杆菌共聚的菌株（ATCC33277和A7436）。这些结果表明，除了众所周知的半乳糖介导的黏附素外，其他黏附素可能参与具核梭杆菌PK1594与牙龈卟啉单胞菌菌株之间的共聚。
