Rosen Graciela, Genzler Tamar, Sela Michael N
Laboratory of Oral Microbiology and Ecology, Faculty of Dental Medicine, The Hebrew University-Hadassah, Jerusalem, Israel.
FEMS Microbiol Lett. 2008 Dec;289(1):59-66. doi: 10.1111/j.1574-6968.2008.01373.x.
Coagreggation of Treponema denticola with either Porphyromonas gingivalis or Fusobacterium nucleatum was characterized and the role of the major outer sheath protein (MSP) in the coaggregation process of these bacteria was evaluated. The MSP of T. denticola was found to be able to bind to P. gingivalis and F. nucleatum cells and this binding could be inhibited by MSP in a concentration-dependent manner. While sodium dodecyl sulfate polyacrylamide gel electrophoresis and Periodic acid-Schiff (PAS) staining of MSP revealed that it is a glycoprotein, monosaccharide analysis showed that MSP contains: Glc (44.4), Gal (20.4%) GlcN (1.3%), GalN (31.6%) and Fuc (9.2%). Peptide N-glycosidase F deglycosylation of MSP was found to inhibit its binding to F. nucleatum but not to P. gingivalis cells. Sugar-binding studies showed that the requirements for the binding of both T. denticola and MSP to F. nucleatum cells are similar to those of the F. nucleatum galactose-binding lectin. These data suggest that MSP acts as an adhesin during the coaggregation process of T. denticola with P. gingivalis and F. nucleatum through its protein and carbohydrate moieties, respectively.
对具核梭杆菌或牙龈卟啉单胞菌与齿垢密螺旋体的共聚作用进行了表征，并评估了主要外鞘蛋白（MSP）在这些细菌共聚过程中的作用。发现齿垢密螺旋体的MSP能够与牙龈卟啉单胞菌和具核梭杆菌细胞结合，并且这种结合能够被MSP以浓度依赖的方式抑制。虽然MSP的十二烷基硫酸钠聚丙烯酰胺凝胶电泳和过碘酸希夫（PAS）染色显示它是一种糖蛋白，但单糖分析表明MSP含有：葡萄糖（44.4%）、半乳糖（20.4%）、氨基葡萄糖（1.3%）、氨基半乳糖（31.6%）和岩藻糖（9.2%）。发现MSP经肽N-糖苷酶F去糖基化后抑制其与具核梭杆菌的结合，但不抑制其与牙龈卟啉单胞菌细胞的结合。糖结合研究表明，齿垢密螺旋体和MSP与具核梭杆菌细胞结合的要求与具核梭杆菌半乳糖结合凝集素的要求相似。这些数据表明，MSP在齿垢密螺旋体与牙龈卟啉单胞菌和具核梭杆菌的共聚过程中分别通过其蛋白质和碳水化合物部分充当黏附素。
