Haghighi Ali, Khorashad Alireza Salimi, Nazemalhosseini Mojarad Ehsan, Kazemi Bahram, Rostami Nejad Mohammad, Rasti Sima
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University, M.C., P.O. Box: 19395-4719, Yeman Street, Chamran Expressway, Tehran, Iran.
Trans R Soc Trop Med Hyg. 2009 May;103(5):452-4. doi: 10.1016/j.trstmh.2008.11.004. Epub 2008 Dec 11.
We investigated the prevalence of intestinal protozoan parasites in patients with gastrointestinal complaints in medical centers in Zahedan, Iran. A total of 1562 stool samples was examined from July 2004 to January 2006 using microscopy (direct smear, formalin-ether concentration), xenic culture and PCR techniques. Four hundred and twenty-seven (27.3%) of the patients were infected with one or more intestinal parasites. Giardia lamblia (10.1%), Entamoeba coli (10%), E. hartmanni (1.7%), Blastocystis hominis (2.2%), Chilomastix mesnili (1.7%), Trichomonas hominis (0.7%), E. histolytica/E. dispar (0.51%) and Iodamoeba butschlii (0.45%) were the most prevalent protozoa detected with microscopy. Of the eight microscopy-positive E. histolytica/E. dispar samples, six were identified as E. dispar by PCR/gel electrophoresis, whereas E. histolytica was not detected at all. Although Zahedan is an area with poor hygiene located in a tropical area near the border of Pakistan and Afghanistan, the prevalence of E. histolytica and E. dispar here compared with other parasites and infectious diseases is unexpectedly low.
我们调查了伊朗扎黑丹医疗中心有胃肠道不适症状患者肠道原生动物寄生虫的感染率。2004年7月至2006年1月期间，共采集了1562份粪便样本，采用显微镜检查（直接涂片、福尔马林-乙醚浓缩法）、虫媒培养和聚合酶链反应（PCR）技术进行检测。427名（27.3%）患者感染了一种或多种肠道寄生虫。通过显微镜检测到的最常见原生动物为蓝氏贾第鞭毛虫（10.1%）、结肠内阿米巴（10%）、哈氏内阿米巴（1.7%）、人芽囊原虫（2.2%）、梅氏唇鞭毛虫（1.7%）、人毛滴虫（0.7%）、溶组织内阿米巴/迪斯帕内阿米巴（0.51%）和布氏嗜碘阿米巴（0.45%）。在显微镜检查呈阳性的8份溶组织内阿米巴/迪斯帕内阿米巴样本中，通过PCR/凝胶电泳鉴定出6份为迪斯帕内阿米巴，未检测到溶组织内阿米巴。尽管扎黑丹是一个卫生条件较差的地区，位于靠近巴基斯坦和阿富汗边境的热带地区，但与其他寄生虫和传染病相比，这里溶组织内阿米巴和迪斯帕内阿米巴的感染率出奇地低。
