Salehi Mitra, Mardaneh Jalal, Niazkar Hamid Reza, Minooeianhaghighi Mohammadhaasan, Arshad Elahe, Soleimani Fateme, Mohammadzadeh Alireza
Department of Parasitology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran.
J Parasitol Res. 2021 Jan 13;2021:8821885. doi: 10.1155/2021/8821885. eCollection 2021.
is the most common intestinal parasite found in humans and many other hosts. Pathogenicity of spp. remains controversial, and it has been suggested that it may be associated with specific subtypes of the organism. This study identified the subtypes and their prevalence rates in the northeast of Iran. A total of 1878 samples were collected from the northeast of Iran from January to December 2017. The patients' demographic details were recorded. Samples were examined by a wet mount, and genomic DNA was extracted from positive samples. Also, PCR was done on the positive samples, and sequencing and phylogenetic analysis were subsequently performed. From 1878 collected stool samples, 152 (8.1%) Blastocystis samples were detected by the microscopic method. Of the 152 samples, spp. were found in 53.6% of the men and 28.9% of the women who showed clinical gastrointestinal symptoms, and a significant relationship was observed between gender and clinical symptoms ( = 0.002). A meaningful relationship was found between the season and infection with this parasite ( value = 0.003). The results of the sequencing of 22 PCR products showed the dominance of ST3, which was isolated from 10 (45.45%) patients, while ST1, ST2, and ST7 were found in 4 (18.19%), 7 (31.81%), and 1 (4.55%) patients, respectively. In this study, ST7 had a low prevalence in the northeast of Iran, and similar to previous studies, ST3 was the dominant subtype.
是人类和许多其他宿主中最常见的肠道寄生虫。 spp.的致病性仍存在争议，有人认为它可能与该生物体的特定亚型有关。本研究确定了伊朗东北部的 亚型及其流行率。2017年1月至12月，共从伊朗东北部采集了1878份样本。记录了患者的人口统计学细节。通过湿涂片检查样本，并从阳性样本中提取基因组DNA。此外，对阳性样本进行了PCR，随后进行了测序和系统发育分析。在1878份采集的粪便样本中，通过显微镜检查法检测到152份（8.1%）芽囊原虫样本。在这152份样本中，出现临床胃肠道症状的男性中有53.6%、女性中有28.9%检测到 spp.，并且观察到性别与临床症状之间存在显著关系（ = 0.002）。发现季节与这种寄生虫感染之间存在有意义的关系（ 值 = 0.003）。22份PCR产物的测序结果显示ST3占主导地位，从10名（45.45%）患者中分离得到，而ST1、ST2和ST7分别在4名（18.19%）、7名（31.81%）和1名（4.55%）患者中发现。在本研究中，ST7在伊朗东北部的流行率较低，与之前的研究相似，ST3是主要亚型。
