Börjesson Stefan, Melin Sara, Matussek Andreas, Lindgren Per-Eric
Department of Clinical and Experimental Medicine, Division of Medical Microbiology, Linköping University, SE-581 85 Linköping, Sweden.
Water Res. 2009 Mar;43(4):925-32. doi: 10.1016/j.watres.2008.11.036. Epub 2008 Dec 6.
The spread of methicillin-resistant Staphylococcus aureus (MRSA), in which the mecA gene mediates resistance, threatens the treatment of staphylococcal diseases. The aims were to determine the effect of wastewater treatment processes on mecA gene concentrations, and the prevalence of S. aureus and MRSA over time. To achieve this a municipal wastewater treatment plant was investigated for the mecA gene, S. aureus and MRSA, using real-time PCR assays. Water samples were collected monthly for one year, at eight sites in the plant, reflecting different aspects of the treatment process. The mecA gene and S. aureus could be detected throughout the year at all sampling sites. MRSA could also be detected, but mainly in the early treatment steps. The presence of MRSA was verified through cultivation from inlet water. The concentration of the mecA gene varied between months and sampling sites, but no obvious seasonal variation could be determined. The wastewater treatment process reduced the mecA gene concentration in most months. Taken together our results show that the mecA gene, S. aureus and MRSA occur over the year at all sites investigated.
耐甲氧西林金黄色葡萄球菌（MRSA）的传播威胁着葡萄球菌疾病的治疗，其中mecA基因介导耐药性。本研究旨在确定废水处理工艺对mecA基因浓度以及金黄色葡萄球菌和MRSA随时间的流行情况的影响。为实现这一目标，使用实时PCR检测法对一座城市污水处理厂的mecA基因、金黄色葡萄球菌和MRSA进行了调查。在一年的时间里，每月在该厂的八个地点采集水样，这些地点反映了处理过程的不同方面。全年在所有采样点均能检测到mecA基因和金黄色葡萄球菌。也能检测到MRSA，但主要在处理的早期阶段。通过对进水进行培养验证了MRSA的存在。mecA基因的浓度在不同月份和采样点有所不同，但未发现明显的季节性变化。在大多数月份，废水处理工艺降低了mecA基因的浓度。综合来看，我们的结果表明，在所调查的所有地点，mecA基因、金黄色葡萄球菌和MRSA全年均有出现。
